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anti-CD14 antibody :: Mouse CD14 Monoclonal Antibody

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Catalog # MBS213102
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

CD14, Monoclonal Antibody

Full Product Name

MOUSE ANTI HUMAN CD14

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
158120
3D Structure
ModBase 3D Structure for P08571
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
UCHM1
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Immunogen
Human Thymocytes followed by peripheral blood mononuclear cells
Histology Positive Control Tissue
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CD14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD14 antibody
Mouse anti Human CD14 antibody, clone UCHM1 recognizes a cell surface antigen of 55 kD, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative  (Hogg et al. 1984). UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.
Applications Tested/Suitable for anti-CD14 antibody
Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-CD14 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.

Testing Data of anti-CD14 antibody
Staining of human peripheral blood monocytes with Mouse anti Human CD14-IgG:Endotoxin Low (MBS224504)
anti-CD14 antibody Testing Data image
Testing Data of anti-CD14 antibody
Staining of human peripheral blood monocytes with Mouse anti Human CD14:FITC (MBS211063)
anti-CD14 antibody Testing Data image
Testing Data of anti-CD14 antibody
Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 (MBS212551) as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone UCHM1 as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively
anti-CD14 antibody Testing Data image
Testing Data of anti-CD14 antibody
Staining of human peripheral blood monocytes with Mouse anti Human CD14:Biotin (MBS210506)
anti-CD14 antibody Testing Data image
Testing Data of anti-CD14 antibody
Staining of human peripheral blood monocytes with Mouse anti Human CD14:Alexa Fluor 647
anti-CD14 antibody Testing Data image
Testing Data of anti-CD14 antibody
Staining of human peripheral blood monocytes with Mouse anti Human CD14:APC (MBS210026)
anti-CD14 antibody Testing Data image
Testing Data of anti-CD14 antibody
Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE (MBS213858)
anti-CD14 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CD14. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,076 Da
NCBI Official Full Name
monocyte differentiation antigen CD14
NCBI Official Synonym Full Names
CD14 molecule
NCBI Official Symbol
NCBI Protein Information
monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
UniProt Protein Name
Monocyte differentiation antigen CD14
UniProt Synonym Protein Names
Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14Cleaved into the following 2 chains:Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form
UniProt Gene Name
UniProt Entry Name
CD14_HUMAN
NCBI Summary for CD14
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
UniProt Comments for CD14
CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; cell surface; extracellular region; plasma membrane; endosome membrane; lipid raft

Molecular Function: peptidoglycan receptor activity; protein binding; opsonin receptor activity; lipopolysaccharide binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; apoptosis; MyD88-independent toll-like receptor signaling pathway; positive regulation of endocytosis; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; phagocytosis; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to magnesium ion; response to ethanol; cell surface receptor linked signal transduction; response to heat; toll-like receptor signaling pathway; innate immune response; inflammatory response; response to electrical stimulus; toll-like receptor 4 signaling pathway; positive regulation of cytokine secretion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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