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anti-CD163 antibody :: Mouse CD163 Monoclonal Antibody

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Catalog # MBS216356
Unit / Price
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  0.25 mg  /  $520 +1 FREE 8GB USB
Product Name

CD163, Monoclonal Antibody

Full Product Name

MOUSE ANTI RAT CD163

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
605545
3D Structure
ModBase 3D Structure for Q86VB7
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ED2
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Histology Positive Control Tissue
Liver
Buffer Solution
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CD163 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD163 antibody
Mouse anti rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007). Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990). Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.
Applications Tested/Suitable for anti-CD163 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-CD163 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul
Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100
Immunohistology - Paraffin: Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase

Testing Data of anti-CD163 antibody
Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power
anti-CD163 antibody Testing Data image
Testing Data of anti-CD163 antibody
Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Medium power
anti-CD163 antibody Testing Data image
Testing Data of anti-CD163 antibody
Staining of acetone fixed, cryostat sectioned rat spleen with Mouse anti Rat CD163 (MBS216354)
anti-CD163 antibody Testing Data image
Testing Data of anti-CD163 antibody
Staining of rat peritoneal macrophages with Mouse anti Rat CD163: RPE (MBS216355)
anti-CD163 antibody Testing Data image
Testing Data of anti-CD163 antibody
Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Medium power
anti-CD163 antibody Testing Data image
Testing Data of anti-CD163 antibody
Staining of rat peritoneal macrophages with Mouse anti Rat CD163: FITC (MBS216353)
anti-CD163 antibody Testing Data image
Testing Data of anti-CD163 antibody
Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. High power
anti-CD163 antibody Testing Data image
Testing Data of anti-CD163 antibody
Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Low power
anti-CD163 antibody Testing Data image
Testing Data of anti-CD163 antibody
Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power
anti-CD163 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CD163. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
124,958 Da
NCBI Official Full Name
CD163
NCBI Official Synonym Full Names
CD163 molecule
NCBI Official Symbol
CD163  [Similar Products]
NCBI Official Synonym Symbols
M130; MM130
  [Similar Products]
NCBI Protein Information
scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen
UniProt Protein Name
Scavenger receptor cysteine-rich type 1 protein M130
UniProt Synonym Protein Names
Hemoglobin scavenger receptor; CD_antigen: CD163Soluble CD163; sCD163
UniProt Gene Name
CD163  [Similar Products]
UniProt Synonym Gene Names
M130; sCD163  [Similar Products]
UniProt Entry Name
C163A_HUMAN
NCBI Summary for CD163
The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
UniProt Comments for CD163
CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: protein binding; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; acute-phase response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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