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anti-CD229 antibody :: Mouse CD229 Monoclonal Antibody

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Catalog # MBS834116
Unit / Price
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  100 Tests  /  $665 +1 FREE 8GB USB
Flow Cytometry (FC/FACS)
Product Name

CD229, Monoclonal Antibody

Full Product Name

CD229 antibody (allophycocyanin)

Product Synonym Names
Monoclonal CD229; Anti-CD229; CD-229; CD229; SLAMF3; Ly9; CD229 (APC); CD 229
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG1
Clone Number
HLy9.25
Host
Mouse
Specificity
Human
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
200 ug/ml (lot specific)
Biological Significance
This HLy9.25 monoclonal antibody reacts with human CD229, an approximately 120-kDa member of the Signaling Lymphocyte Activation Molecule immunoglobulin superfamily. This type I transmembrane glycoprotein is expressed on a variety of immune cell types, including thymocytes, T and B lymphocytes, and natural killer cells. Expression of CD229 is also detected on T and B cell malignancies, including Jurkat and Burkitt lymphoma cells, respectively. CD229 has been shown to interact with SH2-binding phosphatases and the adaptor proteins SLAM-associated protein and EAT-2. Moreover, CD229 localizes to the immunological synapse following T cell activation.
Tag/Conjugate
Allophycocyanin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Other Notes
Small volumes of anti-CD229 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD229 antibody
Mouse monoclonal CD229 antibody (allophycocyanin)
Applications Tested/Suitable for anti-CD229 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD229 antibody
FC: <= 0.05 ug/test

A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Flow Cytometry (FC/FACS) of anti-CD229 antibody
Staining of normal human peripheral blood cells with CD3 antibody PErCP-Cy5.5 and Mouse IgG1 kappa Isotype Control (left) or CD229 antibody (Ly-) (right). Cells in the lymphocyte gate were used for analysis.
anti-CD229 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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