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anti-CD4 antibody :: Mouse CD4 Monoclonal Antibody

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Catalog # MBS213570
Unit / Price
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  100 Tests  /  $450 +1 FREE 8GB USB
Product Name

CD4, Monoclonal Antibody

Full Product Name

MOUSE ANTI HUMAN CD4:RPE

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
186940
Clonality
Monoclonal
Isotype
IgG1
Clone Number
RPA-T4
Host
Mouse
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Other Notes
Small volumes of anti-CD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD4 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD4 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data of anti-CD4 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD4 (MBS212066)
anti-CD4 antibody Testing Data image
Testing Data of anti-CD4 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Alexa Fluor 405 (MBS212066A405)
anti-CD4 antibody Testing Data image
Testing Data of anti-CD4 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:FITC (MBS210641)
anti-CD4 antibody Testing Data image
Testing Data of anti-CD4 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Biotin (MBS210319)
anti-CD4 antibody Testing Data image
Testing Data of anti-CD4 antibody
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 (MBS212066) followed by Histar Detection System. High power
anti-CD4 antibody Testing Data image
Testing Data of anti-CD4 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:RPE
anti-CD4 antibody Testing Data image
Testing Data of anti-CD4 antibody
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 (MBS212066) followed by Histar Detection System. Medium power
anti-CD4 antibody Testing Data image
Testing Data of anti-CD4 antibody
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 (MBS212066) followed by Histar Detection System. Low power
anti-CD4 antibody Testing Data image
Testing Data of anti-CD4 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Alexa Fluor 488 (MBS212066A488)
anti-CD4 antibody Testing Data image
Testing Data of anti-CD4 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Pacific Blue (MBS212066PB)
anti-CD4 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CD4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,111 Da
NCBI Official Full Name
T-cell surface glycoprotein CD4 isoform 1
NCBI Official Synonym Full Names
CD4 molecule
NCBI Official Symbol
NCBI Official Synonym Symbols
CD4mut
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NCBI Protein Information
T-cell surface glycoprotein CD4; CD4 antigen (p55); CD4 receptor; T-cell surface antigen T4/Leu-3
UniProt Protein Name
T-cell surface glycoprotein CD4
UniProt Synonym Protein Names
T-cell surface antigen T4/Leu-3; CD_antigen: CD4
Protein Family
UniProt Gene Name
UniProt Entry Name
CD4_HUMAN
NCBI Summary for CD4
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]
UniProt Comments for CD4
CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; integral to membrane; plasma membrane; external side of plasma membrane; lipid raft

Molecular Function: protein binding; protein homodimerization activity; transmembrane receptor activity; enzyme binding; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding

Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; T cell selection; cytokine production; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; signal transduction; defense response to Gram-negative bacterium; T cell receptor signaling pathway; enzyme linked receptor protein signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; T cell costimulation; protein palmitoleylation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of T cell activation

Disease: Okt4 Epitope Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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