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anti-CD49b antibody :: Mouse CD49b Monoclonal Antibody

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Catalog # MBS216553
Unit / Price DISCONTINUED
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Product Name

CD49b, Monoclonal Antibody

Also Known As

MOUSE ANTI HUMAN CD49b:Biotin

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1181
OMIM
192974
3D Structure
ModBase 3D Structure for P17301
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AK7
Host
Mouse
Form/Format
Biotin
Purified IgG conjugated to Biotin - liquid
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
Buffer Solution
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CD49b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD49b antibody
Mouse anti Human CD49b antibody, clone AK7 recognizes the integrin alpha 2 subunit, which is a 160kD glycoprotein that non-covalently associates with the 130kD integrin beta 1 subunit to form the VLA-2 complex. CD49b is expressed by platelets, long term cultivated T cells, approximately 50% of monocytes and most adherent cell lines. Mouse anti Human CD49b antibody, clone AK7 inhibits cell attachment to collagen.
Applications Tested/Suitable for anti-CD49b antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD49b antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Testing Data of anti-CD49b antibody
Staining of human peripheral blood platelets with Mouse anti Human CD49b:RPE (MBS213872)
anti-CD49b antibody Testing Data image
Testing Data of anti-CD49b antibody
Staining of human peripheral blood monocytes with Mouse anti Human CD49b (MBS213140)
anti-CD49b antibody Testing Data image
Testing Data of anti-CD49b antibody
Staining of human peripheral blood platelets with Mouse anti Human CD49b:FITC (MBS211083)
anti-CD49b antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CD49b. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
129,295 Da
NCBI Official Full Name
integrin alpha-2
NCBI Official Synonym Full Names
integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
NCBI Official Symbol
ITGA2  [Similar Products]
NCBI Official Synonym Symbols
BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
  [Similar Products]
NCBI Protein Information
integrin alpha-2; CD49 antigen-like family member B; VLA2 receptor, alpha-2 subunit; collagen receptor; human platelet alloantigen system 5; platelet antigen Br; platelet glycoprotein GPIa; platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit
UniProt Protein Name
Integrin alpha-2
UniProt Synonym Protein Names
CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; GPIa; VLA-2 subunit alpha; CD_antigen: CD49b
UniProt Gene Name
ITGA2  [Similar Products]
UniProt Synonym Gene Names
CD49B; GPIa  [Similar Products]
UniProt Entry Name
ITA2_HUMAN
NCBI Summary for CD49b
This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
UniProt Comments for CD49b
ITGA2: Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Belongs to the integrin alpha chain family.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: focal adhesion; cell surface; perinuclear region of cytoplasm; plasma membrane; nerve terminal; integrin complex; nucleus; external side of plasma membrane

Molecular Function: viral receptor activity; integrin binding; collagen binding; protein binding; protein heterodimerization activity; metal ion binding; laminin binding

Biological Process: axon guidance; entry of virus into host cell; response to muscle activity; extracellular matrix organization and biogenesis; positive regulation of cell adhesion; positive regulation of positive chemotaxis; positive regulation of translation; cell-matrix adhesion; positive regulation of leukocyte migration; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of collagen binding; positive regulation of cell projection organization and biogenesis; mesodermal cell differentiation; positive regulation of smooth muscle cell migration; response to L-ascorbic acid; response to organic cyclic substance; establishment of protein localization; mammary gland development; positive regulation of smooth muscle contraction; cell adhesion; positive regulation of DNA binding; substrate-bound cell migration; response to drug; integrin-mediated signaling pathway; skin morphogenesis; hypotonic response; cell-substrate adhesion; cellular response to hormone stimulus; detection of mechanical stimulus involved in sensory perception of pain; cell proliferation; organ morphogenesis; response to hypoxia; cell adhesion mediated by integrin; blood coagulation; positive regulation of phagocytosis, engulfment; positive regulation of inflammatory response; response to amine stimulus; positive regulation of transmission of nerve impulse

Disease: Bleeding Disorder, Platelet-type, 9
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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