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anti-CD55 antibody :: Mouse CD55 Monoclonal Antibody

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Catalog # MBS217346
Unit / Price
  0.2 mg  /  $440 +1 FREE 8GB USB
Product Name

CD55, Monoclonal Antibody

Also Known As

MOUSE ANTI HUMAN CD55

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
381
OMIM
125240
3D Structure
ModBase 3D Structure for P08174
Clonality
Monoclonal
Isotype
IgG1
Clone Number
Bu14
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CD55 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD55 antibody
Mouse anti Human CD55 antibody, clone Bu14 recognizes human CD55, also known as Decay Accelerating Factor (DAF). CD55 is a glycosylphosphatylinositol (GPI) anchored type I surface protein which prevents complement mediated lysis of autologous cells. CD55 functions to limit the formation and accelerate the decay of C3/C5-convertases from the classic and alternative pathway. The molecular weight of CD55 varies between 50 and 100kDa dependent on cell type. Alternative transcripts can lead to soluble DAF, which is detectable in plasma, tears, saliva, urine and cerebrospinal fluid. CD55 is the receptor for CD97, Echovirus and Coxsackie B virus, and has been linked to many diseases including multiple sclerosis, rheumatoid arthritis and cancer development.
Applications Tested/Suitable for anti-CD55 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD55 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/200

Testing Data of anti-CD55 antibody
Staining of human peripheral blood monocytes with Mouse anti Human CD55:FITC (MBS217347)
anti-CD55 antibody Testing Data image
Testing Data of anti-CD55 antibody
Staining of human peripheral blood monocytes with Mouse anti Human CD55
anti-CD55 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CD55. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,038 Da
NCBI Official Full Name
complement decay-accelerating factor isoform 1 preproprotein
NCBI Official Synonym Full Names
CD55 molecule, decay accelerating factor for complement (Cromer blood group)
NCBI Official Symbol
NCBI Official Synonym Symbols
CR; TC; DAF; CROM
  [Similar Products]
NCBI Protein Information
complement decay-accelerating factor; CD55 antigen
UniProt Protein Name
Complement decay-accelerating factor
UniProt Synonym Protein Names
CD_antigen: CD55
UniProt Gene Name
UniProt Synonym Gene Names
CR; DAF  [Similar Products]
UniProt Entry Name
DAF_HUMAN
NCBI Summary for CD55
This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]
UniProt Comments for CD55
CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; extracellular region; lipid raft

Molecular Function: viral receptor activity; protein binding; lipid binding

Biological Process: respiratory burst; elevation of cytosolic calcium ion concentration; entry of virus into host cell; negative regulation of complement activation; regulation of complement activation; innate immune response; regulation of lipopolysaccharide-mediated signaling pathway; complement activation, classical pathway

Disease: Blood Group, Cromer System
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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