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anti-CD68 antibody :: Rat CD68 Monoclonal Antibody

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Catalog # MBS215407
Unit / Price
  0.025 mg  /  $165 +1 FREE 8GB USB
Product Name

CD68, Monoclonal Antibody

Popular Item
Also Known As

RAT ANTI MOUSE CD68

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
318
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
FA-11
Host
Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Immunogen
Purified Concanavalin A acceptor glycoprotein from P815 cell line
Buffer Solution
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CD68 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD68 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS)*, In Vitro Assay (IA), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)*
Application Notes for anti-CD68 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm.
Immunohistology - Paraffin: Application Note: This product may require antigen retrieval using heat treatment prior to staining of paraffin sections.Either sodium citrate buffer or Tris/EDTA buffer may be used for this purpose. See Martin-Manso for details. Staining has also been achieved without antigen retrieval, see Lu for details.
Western Blotting: Application Note: Non-reducing conditions recommended.

Testing Data of anti-CD68 antibody
Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 (MBS215395A647). following permeablisation with Leucoperm
anti-CD68 antibody Testing Data image
Testing Data of anti-CD68 antibody
Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 (MBS215395) followed by Goat anti Rat IgG antibody (MBS235195). Low power
anti-CD68 antibody Testing Data image
Testing Data of anti-CD68 antibody
Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 (MBS215395) followed by Goat anti Rat IgG antibody (MBS235195). Medium power
anti-CD68 antibody Testing Data image
Testing Data of anti-CD68 antibody
Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 (MBS215403) with Goat anti Rat IgG:HRP (MBS235195) as a detection antibody
anti-CD68 antibody Testing Data image
Testing Data of anti-CD68 antibody
Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 (MBS215395A488). following permeablisation with Leucoperm
anti-CD68 antibody Testing Data image
Testing Data of anti-CD68 antibody
Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. PLoS ONE 6(11): e28023.
anti-CD68 antibody Testing Data image
Testing Data of anti-CD68 antibody
Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.
anti-CD68 antibody Testing Data image
Testing Data of anti-CD68 antibody
Staining of mouse spleen cryosection with Rat anti Mouse CD68 (MBS215395) followed by Goat anti Rat IgG:HRP (MBS220363) showing staining of macrophages in the red pulp
anti-CD68 antibody Testing Data image
Testing Data of anti-CD68 antibody
Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.
anti-CD68 antibody Testing Data image
Testing Data of anti-CD68 antibody
Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p
anti-CD68 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CD68. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33,844 Da
NCBI Official Synonym Full Names
CD68 antigen
NCBI Official Symbol
NCBI Official Synonym Symbols
Lamp4; gp110; Scard1
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NCBI Protein Information
macrosialin
UniProt Protein Name
Macrosialin
UniProt Synonym Protein Names
CD_antigen: CD68
Protein Family
UniProt Gene Name
UniProt Entry Name
CD68_MOUSE
UniProt Comments for CD68
CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Cellular Component: membrane; lysosome; plasma membrane; integral to membrane; endosome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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