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anti-CD8 ALPHA antibody :: Rat CD8 ALPHA Monoclonal Antibody

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Catalog # MBS213859
Unit / Price
  100 Tests  /  $230 +1 FREE 8GB USB
Product Name

CD8 ALPHA, Monoclonal Antibody

Also Known As

RAT ANTI MOUSE CD8 ALPHA:RPE

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
247
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
KT15
Host
Rat
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. This product should be stored undiluted. DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of reconstitution.
Other Notes
Small volumes of anti-CD8 ALPHA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD8 ALPHA antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD8 ALPHA antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data of anti-CD8 ALPHA antibody
Staining of mouse spleen cells with Rat anti Mouse CD8 (MBS213104)
anti-CD8 ALPHA antibody Testing Data image
Testing Data of anti-CD8 ALPHA antibody
Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG (MBS235195) s a detection reagent. Low power
anti-CD8 ALPHA antibody Testing Data image
Testing Data of anti-CD8 ALPHA antibody
Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE (MBS213860)
anti-CD8 ALPHA antibody Testing Data image
Testing Data of anti-CD8 ALPHA antibody
Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha:RPE
anti-CD8 ALPHA antibody Testing Data image
Testing Data of anti-CD8 ALPHA antibody
Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE - Alexa Fluor 647
anti-CD8 ALPHA antibody Testing Data image
Testing Data of anti-CD8 ALPHA antibody
Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha: Biotin (MBS210507)
anti-CD8 ALPHA antibody Testing Data image
Testing Data of anti-CD8 ALPHA antibody
Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG (MBS235195) s a detection reagent. Medium power
anti-CD8 ALPHA antibody Testing Data image
Testing Data of anti-CD8 ALPHA antibody
Staining of mouse spleen with Rat anti Mouse CD8 Alpha: FITC (MBS211064)
anti-CD8 ALPHA antibody Testing Data image
Testing Data of anti-CD8 ALPHA antibody
Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD8 Alpha: Alexa Fluor 488
anti-CD8 ALPHA antibody Testing Data image
Testing Data of anti-CD8 ALPHA antibody
Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG (MBS235195) s a detection reagent. High power
anti-CD8 ALPHA antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CD8 ALPHA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
27,456 Da
NCBI Official Full Name
T-cell surface glycoprotein CD8 alpha chain isoform 1
NCBI Official Synonym Full Names
CD8 antigen, alpha chain
NCBI Official Symbol
NCBI Official Synonym Symbols
Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
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NCBI Protein Information
T-cell surface glycoprotein CD8 alpha chain; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein Lyt-2
UniProt Protein Name
T-cell surface glycoprotein CD8 alpha chain
UniProt Synonym Protein Names
T-cell surface glycoprotein Lyt-2; CD_antigen: CD8a
UniProt Gene Name
UniProt Synonym Gene Names
Lyt-2; Lyt2  [Similar Products]
UniProt Entry Name
CD8A_MOUSE
UniProt Comments for CD8 ALPHA
CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; protein kinase binding

Biological Process: cell surface receptor linked signal transduction; T cell activation; immune system process; T cell mediated immunity; positive regulation of calcium-mediated signaling; cytotoxic T cell differentiation; defense response to virus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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