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anti-CD8 antibody :: Rat CD8 ALPHA Monoclonal Antibody

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Catalog # MBS214268
Unit / Price
  0.5 mg  /  $540 +1 FREE 8GB USB
Product Name

CD8 ALPHA, Monoclonal Antibody

Also Known As

RAT ANTI MOUSE CD8:Low Endotoxin

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
247
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
YTS105.18
Host
Rat
Form/Format
Low Endotoxin
Purified IgG - liquid
Concentration
IgG concentration 1 mg/ml (lot specific)
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Preparation and Storage
Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD8 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-CD8 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100

Testing Data of anti-CD8 antibody
Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.
anti-CD8 antibody Testing Data image
Testing Data of anti-CD8 antibody
Immunofluorescence staining of a mouse lymph node cryosection with Rat anti mouse CD19, clone 6D5 (MBS212149), green in A and Rat anti Mouse CD8 , red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power
anti-CD8 antibody Testing Data image
Testing Data of anti-CD8 antibody
Staining of mouse spleen cells with Rat anti Mouse CD8 (MBS212026)
anti-CD8 antibody Testing Data image
Testing Data of anti-CD8 antibody
Immunoperoxidase staining of Mouse lymph node cryosection using Rat anti Mouse CD8alpha antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) for detection. Low power
anti-CD8 antibody Testing Data image
Testing Data of anti-CD8 antibody
Immunofluorescence staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody , green in A and Rat anti Mouse CD8 antibody , red in B, Merged image in C
anti-CD8 antibody Testing Data image
Testing Data of anti-CD8 antibody
Published customer image: Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 ug/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistology. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.From: Lacroix-Lamand© S, Rochereau N, Mancassola R, Barrier M, Clauzon A, et al. (2009) Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE 4(12): e8291.
anti-CD8 antibody Testing Data image
Testing Data of anti-CD8 antibody
immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MBS216530), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power
anti-CD8 antibody Testing Data image
Testing Data of anti-CD8 antibody
Published customer image: The effect of NKG2D-blocking antibody on NKG2D-positive CD8+T cells and CD8+T cell accumulation in tumors. 4T-1 tumor -bearing mice were subjected to one of two treatments: Dox plus IL-12 plus control IgG or Dox plus IL-12 plus NKG2D-blocking antibody (n = 3 per treatment). (A) NKG2D expression in CD8+T cells was determined as described for Figure 1 (n.s., not significant). (B) The presence of NKG2D/CD8 -positive cells in tumor sections after the indicated treatments were determined as described for Figure 3.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.
anti-CD8 antibody Testing Data image
Testing Data of anti-CD8 antibody
Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 (MBS216530), green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
anti-CD8 antibody Testing Data image
Testing Data of anti-CD8 antibody
Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 (MBS216530), green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power
anti-CD8 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CD8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
27,456 Da
NCBI Official Full Name
T-cell surface glycoprotein CD8 alpha chain isoform 1
NCBI Official Synonym Full Names
CD8 antigen, alpha chain
NCBI Official Symbol
NCBI Official Synonym Symbols
Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
  [Similar Products]
NCBI Protein Information
T-cell surface glycoprotein CD8 alpha chain; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein Lyt-2
UniProt Protein Name
T-cell surface glycoprotein CD8 alpha chain
UniProt Synonym Protein Names
T-cell surface glycoprotein Lyt-2; CD_antigen: CD8a
UniProt Gene Name
UniProt Synonym Gene Names
Lyt-2; Lyt2  [Similar Products]
UniProt Entry Name
CD8A_MOUSE
UniProt Comments for CD8
CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; protein kinase binding

Biological Process: cell surface receptor linked signal transduction; T cell activation; immune system process; T cell mediated immunity; positive regulation of calcium-mediated signaling; cytotoxic T cell differentiation; defense response to virus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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