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anti-COL4a5 antibody :: Mouse Collagen Type IV Alpha 5 Monoclonal Antibody

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Catalog # MBS2025713 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $110 +1 FREE 8GB USB
  0.05 mg  /  $145 +1 FREE 8GB USB
  0.1 mg  /  $175 +1 FREE 8GB USB
  0.2 mg  /  $230 +1 FREE 8GB USB
  1 mg  /  $515 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Collagen Type IV Alpha 5 (COL4a5), Monoclonal Antibody

Popular Item
Full Product Name

Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)

Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2025713 Technical Datasheet
OMIM
M58526 mRNA
3D Structure
ModBase 3D Structure for P29400
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
D2
Host
Mouse
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/mL (lot specific)
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant COL4a5 (Gly1461~Thr1685) expressed in E.coli
Conjugate
No Conjugate
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2063534
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-COL4a5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-COL4a5 antibody
WB; IHC; ICC; IP.
Application Notes for anti-COL4a5 antibody
Western blotting: 0.5-2ug/mL;1:500-2000
Immunohistochemistry: 5-20ug/mL;1:50-200
Immunocytochemistry: 5-20ug/mL;1:50-200
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-COL4a5 antibody
Western Blot: Sample: Recombinant COL4a5, Human.
anti-COL4a5 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for COL4a5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
collagen alpha-5(IV) chain isoform 1
NCBI Official Synonym Full Names
collagen type IV alpha 5 chain
NCBI Official Symbol
COL4A5  [Similar Products]
NCBI Official Synonym Symbols
ATS; ASLN; CA54
  [Similar Products]
NCBI Protein Information
collagen alpha-5(IV) chain
UniProt Protein Name
Collagen alpha-5(IV) chain
Protein Family
UniProt Gene Name
COL4A5  [Similar Products]
NCBI Summary for COL4a5
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]
UniProt Comments for COL4a5
COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: basal lamina; collagen type IV; endoplasmic reticulum lumen; extracellular region; neuromuscular junction

Molecular Function: extracellular matrix structural constituent

Biological Process: collagen catabolic process; extracellular matrix organization; neuromuscular junction development

Disease: Alport Syndrome, X-linked
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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