• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-DAXX antibody :: Mouse DAXX Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS212521
Unit / Price
Scan QR to view Datasheet
  0.1 mg  /  $300 +1 FREE 8GB USB
anti-DAXX antibody
Product Name

DAXX, Monoclonal Antibody

Popular Item
Full Product Name

MOUSE ANTI HUMAN DAXX

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
603186
3D Structure
ModBase 3D Structure for Q9UER7
Clonality
Monoclonal
Isotype
IgG1
Clone Number
DAXX-01
Host
Mouse
Specificity
Mouse anti Human DAXX antibody, clone DAXX-01 recognizes human Death domain-associated protein 6, also known as DAXX, ETS1-associated protein 1, EAP1 or Fas death domain-associated protein. DAXX is a 740 amino acid ~81 kDa nucleoplasmic protein. DAXX is a regulator of apoptosis implicated in Fas and TGF beta induced apoptosis. DAXX is predominantly a nuclear protein, where it as a repressor of EtsI and Pax3 transactivation. When in association with ASK1 kinase DAXX translocates from the nucleus to the cytoplasm.
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Immunogen
Recombinant C-terminal DAXX protein (aa 558-740)
Buffer Solution
TRIS buffered saline
Target Species
Human
Preparation and Storage
Store at 4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-DAXX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DAXX antibody
MBS212521 recognises the apoptosis related protein DAXX. DAXX is a regulator of apoptosis implicated in Fas and TGF beta induced apoptosis. DAXX is predominantly a nuclear protein, where it as a repressor of EtsI and Pax3 transactivation. When in association with ASK1 kinase DAXX translocates from the nucleus to the cytoplasm.
Applications Tested/Suitable for anti-DAXX antibody
Immunohistology Frozen, Western Blot (WB)
Application Notes for anti-DAXX antibody
Where this antibody has been tested for use in a particular technique this does not necessarily exclude it use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appopriate negative/positive controls.
NCBI/Uniprot data below describe general gene information for DAXX. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
75,563 Da
NCBI Official Full Name
death domain-associated protein 6 isoform a
NCBI Official Synonym Full Names
death-domain associated protein
NCBI Official Symbol
NCBI Official Synonym Symbols
DAP6; EAP1; BING2
  [Similar Products]
NCBI Protein Information
death domain-associated protein 6; CENP-C binding protein; ETS1-associated protein 1; Fas-binding protein; death-associated protein 6; fas death domain-associated protein
UniProt Protein Name
Death domain-associated protein 6
UniProt Synonym Protein Names
Daxx; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein
UniProt Gene Name
UniProt Synonym Gene Names
BING2; DAP6; hDaxx; EAP1  [Similar Products]
UniProt Entry Name
DAXX_HUMAN
NCBI Summary for DAXX
This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]
UniProt Comments for DAXX
DAXX: a transcriptional co-regulatory protein. Proposed to mediate activation of the JNK pathway and apoptosis via ASK1 in response to signaling from FAS and TGF-betaR2. Glucose deprivation activates the ASK1-SEK1-JNK1-HIPK1 pathway, relocalizing Daxx from the nucleus to the cytoplasm, where Daxx binds to ASK1, and subsequently leads to ASK1 oligomerization. Interaction with HSP27 may prevent interaction with TGF-betaR2 and ASK1 and block DAXX-mediated apoptosis. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Two alternatively spliced isoforms have been described.

Protein type: Apoptosis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Nucleolus

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: heterochromatin; PML body; cytoplasm; nucleolus; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein homodimerization activity; histone binding; p53 binding; protein N-terminus binding; protein kinase binding; transcription factor binding; protein binding; enzyme binding; androgen receptor binding; ubiquitin protein ligase binding; heat shock protein binding; receptor signaling protein activity; protein kinase activator activity; transcription corepressor activity

Biological Process: chromatin remodeling; nucleosome assembly; transcription, DNA-dependent; cytokinesis after mitosis; regulation of transcription, DNA-dependent; viral reproduction; induction of apoptosis via death domain receptors; regulation of protein ubiquitination; apoptosis; positive regulation of protein kinase activity; androgen receptor signaling pathway; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; activation of JNK activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000