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anti-E2F1 antibody :: Mouse E2F-1 Monoclonal Antibody

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Catalog # MBS190137
Unit / Price
  0.1 mg  /  $410 +1 FREE 8GB USB
anti-E2F1 antibody
Product Name

E2F-1 (E2F1), Monoclonal Antibody

Also Known As

E2F-1 Monoclonal Antibody

Product Synonym Names
Anti-E2F-1; E2F-1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 20; NC_000020.10 (32263292..32274210, complement). Location: 20q11.2
OMIM
189971
3D Structure
ModBase 3D Structure for Q01094
Clonality
Monoclonal
Isotype
IgG1 kappa
Host
Mouse
Specificity
Anti-E2F-1 [Murine MAb to GST fusion protein corresponding to aa 244-264 of E2F-1, a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter.]
This antibody recognizes an epitope within aa 244-264 of human E2F-1.
Form/Format
100 ug Protein G-purified antibody in PBS, pH 7.4
Antigen
GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.
Stabilizer
None
Preservatives
None. Available on request.
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -70 degree C. Avoid multiple freeze thaw cycles.
Other Notes
Small volumes of anti-E2F1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-E2F1 antibody
Applications Tested/Suitable for anti-E2F1 antibody
Immunoblot
Application Notes for anti-E2F1 antibody
Immunoblotting: use at 1-5 ug/ml.
Immuno precipitation: use at 1-5 ug/ml.
Positive controls: E2F-1 transfectedSoas2 cells and recombinant fusion protein.
NCBI/Uniprot data below describe general gene information for E2F1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,920 Da[Similar Products]
NCBI Official Full Name
E2F-1
NCBI Official Synonym Full Names
E2F transcription factor 1
NCBI Official Symbol
NCBI Official Synonym Symbols
RBP3; E2F-1; RBAP1; RBBP3
  [Similar Products]
NCBI Protein Information
transcription factor E2F1; PBR3; RBAP-1; RBBP-3; OTTHUMP00000030661; PRB-binding protein E2F-1; retinoblastoma-binding protein 3; retinoblastoma-associated protein 1
UniProt Protein Name
Transcription factor E2F1
UniProt Synonym Protein Names
PBR3; PRB-binding protein E2F-1; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
RBBP3  [Similar Products]
UniProt Entry Name
E2F1_HUMAN
NCBI Summary for E2F1
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq]
UniProt Comments for E2F1
E2F1: a member of the E2F/DP family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Binds DNA cooperatively with Dp transcription factors in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.The Dp-1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. It can mediate both cell proliferation and p53-dependent apoptosis.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: nucleoplasm; Rb-E2F complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity

Biological Process: Notch signaling pathway; transcription, DNA-dependent; apoptosis; positive regulation of transcription, DNA-dependent; mRNA stabilization; negative regulation of transcription from RNA polymerase II promoter; anoikis; positive regulation of fibroblast proliferation; regulation of transcription, DNA-dependent; DNA damage response, signal transduction resulting in induction of apoptosis; DNA damage checkpoint; forebrain development; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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