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anti-ESR2 antibody :: Mouse ESTROGEN RECEPTOR BETA 1 Monoclonal Antibody

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Catalog # MBS210532
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Product Name

ESTROGEN RECEPTOR BETA 1 (ESR2), Monoclonal Antibody

Popular Item
Full Product Name

MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 1

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601663
3D Structure
ModBase 3D Structure for Q92731
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
PPG5/10
Host
Mouse
Form/Format
Concentrated Tissue Culture Supernatant - liquid
Perservative Stabilisers
0.1% Sodium Azide (NaN3)
Histology Positive Control Tissue
Ovary
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-ESR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ESR2 antibody
Mouse anti Human estrogen receptor beta 1, clone PPG5/10 specifically recognizes the estrogen receptor beta 1 (ERB1), one of several isoforms of estrogen receptor (ER) which can be used in conjunction with progesterone receptor (PR) and HER2/neu (CD340), to help determine both prognosis and the effectiveness of hormonal therapy, in breast cancer patients. Prognosis and survival rate varies greatly depending on cancer type, stage and treatment.Mouse anti Human estrogen receptor beta 1, clone PPG5/10 is increasingly being utilized in the immunohistochemical staining of formal fixed paraffin embedded tissue, in preference to ligand binding assays, for the detection of ER/PR. ER/PR is present in approximately 75% to 80% of breast tumors, and breast cancer cells with these receptors depend on the hormones estrogen and/or progesterone to grow.Mouse anti Human estrogen receptor beta 1, clone PPG5/10 can be used to demonstrate the nuclear presence of ERB1 within cells of the breast (see Shaaban, A.M.et al. for details), ovary, uterus, oviduct, testis, prostate, bladder and male reproductive ducts. Clone PPG5/10 has also been reported to stain cells in the adenohypophysis, demonstrating cytoplasmic expression. These results await further characterization.Clone PPG5/10 is reported as suitable for use in Western blotting, see Shaaban, A.M.et al. for details
Applications Tested/Suitable for anti-ESR2 antibody
Immunohistology Frozen, Immunofluorescence (IF), Immunohistology Paraffin*
Application Notes for anti-ESR2 antibody
Immunohistology - Paraffin: Minimum Dilution: 1/5; Maximum Dilution: 1/50; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
0.05M glycine/EDTA pH8 see (See Carpino et al.) or sodium citrate buffer pH 6.0 are recommended for this purpose.

Testing Data of anti-ESR2 antibody
Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta 1 antibody (MBS214107) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power)
anti-ESR2 antibody Testing Data image
Testing Data of anti-ESR2 antibody
Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta 1 antibody (MBS214107) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power)
anti-ESR2 antibody Testing Data image
Testing Data of anti-ESR2 antibody
Published customer image: Expression of PR paralleled that of ERa not ERbeta. A. Expression of PR mRNA was significantly higher in cancers that were classified as well or moderately differentiated as compared with those with a poorly differentiated phenotype (p < 0.05, n = 10 cancers in each group, comparisons indicated by letters a, b). B. PR mRNA was significantly higher in Ishikawa A [ERa-positive] compared to Ishikawa B [ERa-negative] after incubation with E2 for 16 (a) or 24 (b) hours (p < 0.01). Values are expressed as mean +/- SD of three independent experiments performed in duplicate. C. Fluorescent co-localisation as carried out using antibodies specific for ERa or ERbeta1 (both green) and PR (red). The cancers illustrated were classified as well (code 1614), moderately (code 1930) or poorly (c, codes 0001 and 1176) differentiated; at least 8 samples were analysed in each group. Co-expression was detected as yellow/orange immunofluoresence. In the well and moderately differentiated cancers expression of PR was most intense in epithelial cells and broadly overlapped with that of ERa (e.g. in cells indicated by arrows). Expression of PR was very low in the poorly differentiated cancers and appeared confined to cells with a fibroblast phenotype (**). Some ERbeta1 positive cells were PR positive however most cells in the poorly differentiated cancers were ERbeta1 positive and PR negative (green nuclei). Labels: L = lumen, S = stromal compartment, arrowheads = ERbeta1 postive cells that are PR negative.From: Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, Jabbour HN, Saunders PT. Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha. BMC Cancer. 2009 Sep 16;9:330.
anti-ESR2 antibody Testing Data image
Testing Data of anti-ESR2 antibody
Staining of paraffin embedded human breast cancer tissue with Mouse anti Human estrogen receptor (MBS215424) following antigen retrieval with EDTA. Detection was performed with Histar detection kit (MBS239184)
anti-ESR2 antibody Testing Data image
Testing Data of anti-ESR2 antibody
Staining of formalin fixed, paraffin embedded human placenta with Mouse anti Human estrogen receptor (MBS214107)
anti-ESR2 antibody Testing Data image
Testing Data of anti-ESR2 antibody
Published customer image: Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. All proteins were immunolocalised to cell nuclei (see higher power inserts in panels e, f, c and d respectively). In the well and moderately differentiated cancers expression was most intense in epithelial cell layers (arrowheads, panel A and inserts). Note that ERa was low/absent in poor grade cancers (J) but immunoexpression of ERbeta1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERbeta1, ERbeta2 and ERbeta5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers.From: Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, Jabbour HN, Saunders PT. Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha. BMC Cancer. 2009 Sep 16;9:330.
anti-ESR2 antibody Testing Data image
Testing Data of anti-ESR2 antibody
Staining of paraffin embedded human breast cancer tissue with Mouse anti Human estrogen receptor (MBS214107) following antigen retrieval with EDTA. Detection was performed with Histar detection kit (MBS239184)
anti-ESR2 antibody Testing Data image
Testing Data of anti-ESR2 antibody
Staining of formalin fixed, paraffin embedded human placenta with Mouse anti Human estrogen receptor (MBS214107)
anti-ESR2 antibody Testing Data image
Testing Data of anti-ESR2 antibody
Photomicrograph of human ovary immunostained for estrogen receptor beta using monoclonal antibody MBS215425
anti-ESR2 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for ESR2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53,209 Da[Similar Products]
NCBI Official Full Name
estrogen receptor beta isoform 2
NCBI Official Synonym Full Names
estrogen receptor 2 (ER beta)
NCBI Official Symbol
NCBI Official Synonym Symbols
Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
  [Similar Products]
NCBI Protein Information
estrogen receptor beta; estrogen receptor beta 4; nuclear receptor subfamily 3 group A member 2
UniProt Protein Name
Estrogen receptor beta
UniProt Synonym Protein Names
Nuclear receptor subfamily 3 group A member 2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ESTRB; NR3A2; ER-beta  [Similar Products]
UniProt Entry Name
ESR2_HUMAN
NCBI Summary for ESR2
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for ESR2
ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: nucleoplasm; mitochondrion; extracellular region; nucleus

Molecular Function: receptor antagonist activity; protein binding; ligand-dependent nuclear receptor activity; enzyme binding; estrogen receptor activity; DNA binding; zinc ion binding; transcription coactivator activity; estrogen response element binding; steroid hormone receptor activity; transcription factor activity; steroid binding

Biological Process: transcription initiation from RNA polymerase II promoter; estrogen receptor signaling pathway; induction of apoptosis by hormones; neuron migration; vagina development; negative regulation of transcription from RNA polymerase II promoter; uterus development; signal transduction; regulation of transcription, DNA-dependent; cell-cell signaling; ovarian follicle development; gene expression; steroid hormone mediated signaling; positive regulation of transcription factor activity; negative regulation of cell growth; brain development; negative regulation of epithelial cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Organs/Tissues associated with anti-ESR2 antibody
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