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anti-F4/80 antibody :: Rat F4/80 Monoclonal Antibody

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Catalog # MBS216411
Unit / Price
  0.025 mg  /  $165 +1 FREE 8GB USB
Product Name

F4/80, Monoclonal Antibody

Full Product Name

RAT ANTI MOUSE F4/80:Biotin

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
Cl:A3-1
Host
Rat
Form/Format
Biotin
Purified IgG conjugated to Biotin - liquid
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-F4/80 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-F4/80 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-F4/80 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data of anti-F4/80 antibody
Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC (MBS216414)
anti-F4/80 antibody Testing Data image
Testing Data of anti-F4/80 antibody
Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 (MBS216424), red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria
anti-F4/80 antibody Testing Data image
Testing Data of anti-F4/80 antibody
Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC (MBS216408)
anti-F4/80 antibody Testing Data image
Testing Data of anti-F4/80 antibody
Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750 (MBS219371P750)
anti-F4/80 antibody Testing Data image
Testing Data of anti-F4/80 antibody
Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin (MBS216412)
anti-F4/80 antibody Testing Data image
Testing Data of anti-F4/80 antibody
Frozen mouse spleen stained with A: Rat anti Mouse F4/80 (MBS219371) followed by Goat anti Rat IgG:HRP (MBS235195) or B: Rat anti Mouse F4/80 (MBS219371) preincubated with 2 molar excess of Human anti Idiotypic MBS219371 (HCA154) followed by Goat anti Rat IgG:HRP (MBS235195)
anti-F4/80 antibody Testing Data image
Testing Data of anti-F4/80 antibody
Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin (MBS216409)
anti-F4/80 antibody Testing Data image
Testing Data of anti-F4/80 antibody
Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue(MBS219371PB)
anti-F4/80 antibody Testing Data image
Testing Data of anti-F4/80 antibody
Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P
anti-F4/80 antibody Testing Data image
Testing Data of anti-F4/80 antibody
Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin (MBS216410)
anti-F4/80 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for F4/80. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
102,130 Da
NCBI Official Full Name
EGF-like module-containing mucin-like hormone receptor-like 1
NCBI Official Synonym Full Names
EGF-like module containing, mucin-like, hormone receptor-like sequence 1
NCBI Official Symbol
NCBI Official Synonym Symbols
Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
  [Similar Products]
NCBI Protein Information
EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; cell surface glycoprotein F4/80; lymphocyte antigen 71
UniProt Protein Name
EGF-like module-containing mucin-like hormone receptor-like 1
UniProt Synonym Protein Names
Cell surface glycoprotein F4/80; EGF-like module receptor 1; EMR1 hormone receptor
UniProt Gene Name
UniProt Synonym Gene Names
Gpf480  [Similar Products]
UniProt Entry Name
EMR1_MOUSE
UniProt Comments for F4/80
EMR1: Could be involved in cell-cell interactions. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; GPCR, family 2; Receptor, GPCR; Membrane protein, integral

Cellular Component: membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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