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anti-F8 antibody :: Mouse FACTOR VIII Monoclonal Antibody

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Catalog # MBS219502
Unit / Price
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  0.2 mg  /  $430 +1 FREE 8GB USB
Testing Data
Product Name

FACTOR VIII (F8), Monoclonal Antibody

Popular Item
Full Product Name

MOUSE ANTI HUMAN FACTOR VIII

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
134500
3D Structure
ModBase 3D Structure for P00451
Clonality
Monoclonal
Isotype
IgG1
Clone Number
RFF-VIIIC/5
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Immunogen
Affinity purified human Factor VIII
Buffer Solution
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-F8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-F8 antibody
Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/5 recognizes human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterised by the body's inability to control blood clotting. This could result in severe blood loss, even with minor injuries. Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/5 recognizes an epitope towards the C-terminus of full-length Factor VIII. It also recognizes the 80kDa and 70kDa cleavage products.
Applications Tested/Suitable for anti-F8 antibody
ELISA (EIA), Radioimmunoassays (RIA), Western Blot (WB)
Application Notes for anti-F8 antibody
ELISA: Minimum Dilution: 1/400; Maximum Dilution: 1/4000

Testing Data of anti-F8 antibody
Recombinant Human Factor VIII detected with Mouse anti Human Factor VIII (MBS219440)
anti-F8 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for F8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,641 Da[Similar Products]
NCBI Official Full Name
coagulation factor VIII isoform a
NCBI Official Synonym Full Names
coagulation factor VIII, procoagulant component
NCBI Official Symbol
NCBI Official Synonym Symbols
AHF; F8B; F8C; HEMA; FVIII; DXS1253E
  [Similar Products]
NCBI Protein Information
coagulation factor VIII; antihemophilic factor; coagulation factor VIIIc; factor VIII F8B
UniProt Protein Name
Coagulation factor VIII
UniProt Synonym Protein Names
Antihemophilic factor; AHF; Procoagulant componentCleaved into the following 4 chains:Factor VIIIa heavy chain, 200 kDa isoform; Factor VIIIa heavy chain, 92 kDa isoform; Factor VIII B chain; Factor VIIIa light chain
UniProt Gene Name
UniProt Synonym Gene Names
F8C; AHF  [Similar Products]
UniProt Entry Name
FA8_HUMAN
NCBI Summary for F8
This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]
UniProt Comments for F8
F8: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. Defects in F8 are the cause of hemophilia A (HEMA). A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non- functional; i.e. the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. Belongs to the multicopper oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: protein binding; copper ion binding; serine-type endopeptidase activity; oxidoreductase activity

Biological Process: platelet activation; platelet degranulation; acute-phase response; blood coagulation; proteolysis; blood coagulation, intrinsic pathway

Disease: Hemophilia A; Factor Viii Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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