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anti-GFP antibody :: Mouse GFP Monoclonal Antibody

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Catalog # MBS533341
Unit / Price
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  0.1 mL  /  $395 +1 FREE 8GB USB
Product Name

GFP, Monoclonal Antibody

Full Product Name

GFP antibody

Product Synonym Names
Monoclonal GFP; Anti-GFP; Green Fluorescent Protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
4B10B2
Host
Mouse
Form/Format
Purified antibody supplied in PBS containing 0.03% sodium azide.
Concentration
1.0 mg/ml (lot specific)
Biological Significance
Green Fluorescent Protein (GFP) tagging provide an excellent means for monitoring gene expression and protein localization in living cells, so that it is widely accepted by molecular and cell biological research. Monoclonal anti-GFP antibody can detect GFP and its variants on Western blotting, Immunoprecipitation and Immunocytochemistry.
Immunogen
GFP antibody was raised in Mouse using a purified recombinant fragment of GFP expressed in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-GFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GFP antibody
Mouse monoclonal GFP antibody
Applications Tested/Suitable for anti-GFP antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-GFP antibody
ELISA: 1:10,000
FC: 1:200-1:400
IHC: 1:200-1:1000
WB: 1:500-1:2000

Western Blot (WB) of anti-GFP antibody
Western Blot showing GFP antibody used against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).
anti-GFP antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-GFP antibody
Western Blot showing using GFP antibody used against extracts from HCC827 cells, untransfected (1) and transfected with GFP (2).
anti-GFP antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-GFP antibody
Immunocytochemistry analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP antibody.
anti-GFP antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for GFP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI Official Full Name
GFP
Protein Family
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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