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anti-GAPDH antibody :: Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Monoclonal Antibody

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Catalog # MBS312511
Unit / Price
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  1 mg  /  $1,125 +1 FREE 8GB USB
anti-GAPDH antibody
Product Name

Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH), Monoclonal Antibody

Full Product Name

MAb to GAPDH

Product Synonym Names
Monoclonal Antibody to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 12; NC_000012.11 (6643657..6647536). Location: 12p13
OMIM
138400
3D Structure
ModBase 3D Structure for P04406
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4G5
Host
Host: Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells from BALB/c mice.
Source: Ascites
Specificity
Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH)
Recognizes GAPDH monomer (36kDa) and the dimer form. Does not react with the tetrameric form.
Purity/Purification
>90% pure (SDS-PAGE). Protein A chromatography
Form/Format
HRP, Liquid
Concentration
0.9mg/ml (Sigma protein assay kit) (lot specific)
Immunogen
Rabbit muscle GAPDH
Affinity Constant
Not determined
Buffer
PBS, pH 7.4
Preservative
0.05% ProClin 300
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Warnings
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Preparation and Storage
Store at 2 to 8 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-GAPDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
36,053 Da[Similar Products]
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH  [Similar Products]
NCBI Official Synonym Symbols
G3PD; GAPD; MGC88685
  [Similar Products]
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase; OTTHUMP00000174430; OTTHUMP00000174431; OTTHUMP00000174432; OTTHUMP00000174434; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Synonym Protein Names
Peptidyl-cysteine S-nitrosylase GAPDH
UniProt Gene Name
GAPDH  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
G3P_HUMAN
NCBI Summary for GAPDH
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
UniProt Comments for GAPDH
Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC

By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Ref.6 Ref.23

Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. Ref.6

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure: Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation

By similarity. Interacts with EIF1AD, USP25, PRKCI and WARS. Ref.23 Ref.25 Ref.28 Ref.36 Ref.39 Ref.40

Subcellular location: Cytoplasm › cytosol. Nucleus

By similarity. Cytoplasm › perinuclear region. Membrane. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal

By similarity. Postnuclear and Perinuclear regions. Ref.24

Post-translational modification: S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus

By similarity.ISGylated

Probable. Ref.29

Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Product References and Citations for anti-GAPDH antibody
• FEBS Letters, (1992), Volume 300, number 1, 9-12.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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