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anti-HK2 antibody :: Mouse HEXOKINASE 1 & 2 Monoclonal Antibody

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Catalog # MBS219117
Unit / Price
  0.1 mg  /  $445 +1 FREE 8GB USB
Testing Data
Product Name

HEXOKINASE 1 & 2 (HK2), Monoclonal Antibody

Also Known As

MOUSE ANTI HUMAN HEXOKINASE 1 & 2

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
917
OMIM
601125
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1A7
Host
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Perservative Stabilisers
0.1% Sodium Azide (NaN3)
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-HK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HK2 antibody
Mouse anti Human Hexokinase 1&2, clone 1A7 recognizes human hexokinase 1 (HK1) and hexokinase 2 (HK2), 102kDa allosteric enzymes of the glycolytic pathway, which catalyse the phosphorylation of glucose to form glucose-6-phosphate (Glc-6-P). Hexokinase 1 is predominantly expressed in the brain while Hexokinase 2 is predominantly expressed in skeletal muscle. Both are members of four distinct mammalian hexokinase isozymes (hexokinase type I to IV), which localize to the outer membrane of mitochondria and is regulated (inhibited) by its product Glc-6-P (Wolf et al. 2011)
Applications Tested/Suitable for anti-HK2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-HK2 antibody
Western Blot: This item detects a band of approximately 102 kDa in Jurkat cell lysates.
Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/3000

Testing Data of anti-HK2 antibody
Western blotting analysis of hexokinase specificity using Mouse anti Human hexokinase 1&2, clone 1A7 A: hexokinase specificity against recombinant hexokinase proteinsB: hexokinase expression in Jurkat cell lysate
anti-HK2 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for HK2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
102,380 Da[Similar Products]
NCBI Official Full Name
hexokinase-2
NCBI Official Synonym Full Names
hexokinase 2
NCBI Official Symbol
NCBI Official Synonym Symbols
HKII; HXK2
  [Similar Products]
NCBI Protein Information
hexokinase-2; HK II; hexokinase type II; hexokinase-2, muscle; muscle form hexokinase
UniProt Protein Name
Hexokinase-2
UniProt Synonym Protein Names
Hexokinase type II; HK II; Muscle form hexokinase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
HK II  [Similar Products]
UniProt Entry Name
HXK2_HUMAN
NCBI Summary for HK2
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]
UniProt Comments for HK2
HK2: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). The predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Genetic variations of HK2 do not appear to contribute to NIDDM.

Protein type: Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.1.1; Kinase, other; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - fructose and mannose; Mitochondrial

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: mitochondrial outer membrane; membrane; cytosol

Molecular Function: hexokinase activity; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding; glucose binding

Biological Process: lactation; apoptotic mitochondrial changes; glycolysis; hexose transport; carbohydrate metabolic process; carbohydrate phosphorylation; glucose 6-phosphate metabolic process; cell glucose homeostasis; glucose metabolic process; pathogenesis; regulation of glucose import; glucose transport; transmembrane transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-HK2 antibody
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