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anti-MCP-1 antibody :: Mouse Macrophage/Monocyte Chemotactic & Activating Factor Monoclonal Antibody

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Catalog # MBS140020
Unit / Price
  0.5 mg  /  $305 +1 FREE 8GB USB
  1 mg  /  $455 +1 FREE 8GB USB
anti-MCP-1 antibody
Product Name

Macrophage/Monocyte Chemotactic & Activating Factor (MCP-1), Monoclonal Antibody

Also Known As

Mouse Anti Human Macrophage/Monocyte Chemotactic & Activating Factor

Product Synonym Names
MCP 1 Antibody; Macrophage/Monocyte Chemotactic & Activating Factor, Mouse Anti-Human; Small inducible cytokine A2; CCL2; Monocyte chemotactic protein 1; MCP-1; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte secretory protein JE; HC11; chemokine (C-C motif) ligand 2; MCP1; SCYA2; GDCF-2; SMC-CF; HSMCR30; MGC9434; GDCF-2 HC11
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
99
OMIM
158105
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
YNR-HMCAF
Host
Mouse
Purity/Purification
Ion exchange.
Concentration
1mg/ml in PBS (after reconstitution) Shipping ConditionsAntibody is shipped lyophilized at ambient temperature. (lot specific)
Reconstitution
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Note
MCAF does not bind to plastic as good as most proteins - use higher concentration for proper binding.
Immunogen
r.Human MCAF.
Preparation and Storage
In lyophilized form, for long periods, store at 4 degree C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20 degree C.
Other Notes
Small volumes of anti-MCP-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MCP-1 antibody
Introduction: Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor ?B (NF?B). CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weight of approximately 13kDa. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5.
Applications Tested/Suitable for anti-MCP-1 antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-MCP-1 antibody
Direct ELISA, Western Blot, Immunoprecipitation.
In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories) 1:5,000 dilution will give 0.3 O.D within 10 minutes.
NCBI/Uniprot data below describe general gene information for MCP-1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,025 Da
NCBI Official Full Name
C-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 2
NCBI Official Symbol
NCBI Official Synonym Symbols
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
  [Similar Products]
NCBI Protein Information
C-C motif chemokine 2; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte chemotactic protein 1; monocyte secretory protein JE; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small-inducible cytokine A2
UniProt Protein Name
C-C motif chemokine 2
UniProt Synonym Protein Names
HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; MCP-1; Monocyte secretory protein JE; Small-inducible cytokine A2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
MCP1; SCYA2; MCAF; MCP-1  [Similar Products]
UniProt Entry Name
CCL2_HUMAN
NCBI Summary for MCP-1
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
UniProt Comments for MCP-1
CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Chemokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; rough endoplasmic reticulum; endocytic vesicle; perinuclear region of cytoplasm; dendrite; extracellular region; synapse; perikaryon; nerve terminal

Molecular Function: heparin binding; CCR2 chemokine receptor binding; chemokine activity; receptor binding; protein kinase activity

Biological Process: maternal process involved in pregnancy; protein amino acid phosphorylation; response to antibiotic; monocyte chemotaxis; regulation of cell shape; cell surface receptor linked signal transduction; transforming growth factor beta receptor signaling pathway; response to vitamin B3; cellular homeostasis; negative regulation of neuron apoptosis; cell adhesion; neutrophil chemotaxis; organ regeneration; response to amino acid stimulus; positive regulation of tumor necrosis factor production; JAK-STAT cascade; G-protein signaling, coupled to cyclic nucleotide second messenger; organ morphogenesis; response to ethanol; unfolded protein response, activation of signaling protein activity; response to bacterium; cellular response to insulin stimulus; response to heat; response to mechanical stimulus; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; signal transduction; chemotaxis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; protein kinase B signaling cascade; response to wounding; lipopolysaccharide-mediated signaling pathway; response to gamma radiation; angiogenesis; inflammatory response; lymphocyte chemotaxis; aging; unfolded protein response; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; viral genome replication; macrophage chemotaxis; leukocyte migration during inflammatory response; humoral immune response; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; positive regulation of leukocyte mediated cytotoxicity; cellular protein metabolic process; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration

Disease: Neural Tube Defects; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To
Precautions
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Disclaimer
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