• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-MMP9 antibody :: Mouse Matrix Metalloproteinase 9 Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS2025637 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $90 +1 FREE 8GB USB
  0.02 mg  /  $100 +1 FREE 8GB USB
  0.05 mg  /  $130 +1 FREE 8GB USB
  0.1 mg  /  $160 +1 FREE 8GB USB
  0.2 mg  /  $200 +1 FREE 8GB USB
  1 mg  /  $430 +1 FREE 8GB USB
Product Name

Matrix Metalloproteinase 9 (MMP9), Monoclonal Antibody

Popular Item
Full Product Name

Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)

Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2025637 Technical Datasheet
OMIM
J05070 mRNA
3D Structure
ModBase 3D Structure for P14780
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
16#
Host
Mouse
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1 mg/ml (lot specific)
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant MMP9 (Gly213~Ala399) expressed in E.coli
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-MMP9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-MMP9 antibody
Applications Tested/Suitable for anti-MMP9 antibody
WB; IHC; ICC; IP.
Application Notes for anti-MMP9 antibody
Western blotting: 0.5-5 ug/mL;1:200-2000
Immunohistochemistry: 5-20 ug/mL;1:50-200
Immunocytochemistry: 5-20 ug/mL;1:50-200

Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-MMP9 antibody
Western Blot: Sample: Human U937 cell lysate; Primary Ab: 3ug/ml Mouse Anti-Human MMP9 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (#MBS2086035)
anti-MMP9 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-MMP9 antibody
Western Blot: Sample: Recombinant MMP9, Human.
anti-MMP9 antibody Western Blot (WB) (WB) image
Knockout Validation of anti-MMP9 antibody
Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: MMP9 knockout HepG2 cell lysate; Predicted MW: 76kDa Observed MW: 90kDa Primary Ab: 3ug/ml Mouse Anti-Human MMP9 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (#MBS2086035)
anti-MMP9 antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for MMP9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
matrix metalloproteinase-9 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 9
NCBI Official Symbol
NCBI Official Synonym Symbols
GELB; CLG4B; MMP-9; MANDP2
  [Similar Products]
NCBI Protein Information
matrix metalloproteinase-9
UniProt Protein Name
Matrix metalloproteinase-9
UniProt Synonym Protein Names
92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CLG4B; MMP-9; GELB  [Similar Products]
NCBI Summary for MMP9
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]
UniProt Comments for MMP9
MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.35; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular exosome; extracellular region; extracellular space; ficolin-1-rich granule lumen; proteinaceous extracellular matrix; tertiary granule lumen

Molecular Function: collagen binding; endopeptidase activity; identical protein binding; metalloendopeptidase activity; metallopeptidase activity; protein binding; serine-type endopeptidase activity; zinc ion binding

Biological Process: cellular response to cadmium ion; cellular response to reactive oxygen species; collagen catabolic process; embryo implantation; endodermal cell differentiation; ephrin receptor signaling pathway; extracellular matrix disassembly; leukocyte migration; macrophage differentiation; negative regulation of apoptosis; negative regulation of cation channel activity; negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway; negative regulation of intrinsic apoptotic signaling pathway; neutrophil degranulation; ossification; positive regulation of cell migration; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; positive regulation of protein phosphorylation; positive regulation of receptor binding; positive regulation of release of cytochrome c from mitochondria; positive regulation of vascular smooth muscle cell proliferation; proteolysis; skeletal system development

Disease: Metaphyseal Anadysplasia 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000