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anti-MBD3 antibody :: Mouse MBD3 Monoclonal Antibody

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Catalog # MBS831032
Unit / Price DISCONTINUED
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anti-MBD3 antibody
Product Name

MBD3, Monoclonal Antibody

Also Known As

MBD3 antibody

Product Synonym Names
Monoclonal MDB3; Anti-MDB3; MBD-3; MBD 3; methyl-CpG binding Domain Protein 3; MBD3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
259
OMIM
603573
3D Structure
ModBase 3D Structure for O95983
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3A3
Host
Mouse
Specificity
Human
Purity/Purification
MBD3 antibody was affinity purified with antigen peptides
Form/Format
Lyophilized product from 1% BSA in PBS containing 0.05% NaN3, reconstitute with 1 ml of deionised water
Source Note
Supernatant
Biological Significance
Methylation at CpG dinucleotides in genomic DNA is a fundamental epigenetic mechanism of gene expression control in vertebrates. Proteins with a methyl-CpG binding domain (MBD) can bind to single methylated CpGs and most of them are involved in transcription control. So far, five vertebrate MBD proteins have been described as MBD family members: BD1, MBD2, MBD3, MBD4 and MECP2.
Immunogen
MBD3 antibody was raised in rabit using a synthetic peptide corresponding to part of the C terminal of human MBD3 (EELARDGEAPLDKAC) as the immunogen
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at 20 degree C for long term storage
Other Notes
Small volumes of anti-MBD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MBD3 antibody
Human MBD3 antibody
Product Categories/Family for anti-MBD3 antibody
Applications Tested/Suitable for anti-MBD3 antibody
Western Blot (WB)
Application Notes for anti-MBD3 antibody
WB: 0.1 ug/ml
NCBI/Uniprot data below describe general gene information for MBD3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,014 Da
NCBI Official Synonym Full Names
methyl-CpG binding domain protein 3
NCBI Official Symbol
NCBI Protein Information
methyl-CpG-binding domain protein 3
UniProt Protein Name
Methyl-CpG-binding domain protein 3
UniProt Synonym Protein Names
Methyl-CpG-binding protein MBD3
UniProt Gene Name
UniProt Entry Name
MBD3_HUMAN
NCBI Summary for MBD3
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
UniProt Comments for MBD3
MBD3: methyl-CpG binding (MBD) proteins appear to bind differentially methylated domains (DMDs) of DNA, to recruit repression complexes, and to silence the locus. The methyl CpG binding domain of human MBD3 may not directly bind mCpG but does interact with the NuRD/Mi2 components HDAC1 and MTA2. Plays a role in histone deacetylation and nucleosome remodeling. Increased levels have been reported in lung cancer and glioblastomas and may be a therapeutic target.

Protein type: Gene silencing; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; heterochromatin; protein complex; nuclear chromatin; cytoplasm; NuRD complex

Molecular Function: protein binding; nucleosomal DNA binding; DNA binding; methyl-CpG binding

Biological Process: tissue development; establishment and/or maintenance of chromatin architecture; methylation-dependent chromatin silencing; transcription, DNA-dependent; in utero embryonic development; ATP-dependent chromatin remodeling; negative regulation of transcription from RNA polymerase II promoter; histone acetylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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