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Synaptopodin Monoclonal Antibody

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Catalog # MBS313029
Unit / Price
  0.05 mg  /  $745 +1 FREE 8GB USB
Western Blot
Product Name

Synaptopodin, Monoclonal Antibody

Popular Item
Full Product Name

MAb to Synaptopodin

Product Synonym Names
Monoclonal Antibody to Synaptopodin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG1
Clone Number
G1D4
Host
Host: Mouse
Source: Cell Culture
Specificity
Recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of rat brain. Differentiation-dependent expression in cultured hippocampal neurons. Reacts specifically with synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Cross-reacts with human, bovine, rat, mouse, guinea pig and gerbil. Does not cross-react with rabbit, frog or chicken
Purity/Purification
Protein A chromatography
Form/Format
Purified, Lyophilized. Reconstitute with 1ml distilled water.
Concentration
50ug/ml (prior to lyophilization) (lot specific)
Immunogen
Isolated rat kidney glomeruli
Affinity Constant
None
Buffer
Lyophilized from PBS, pH 7.4 containing 0.5% BSA
Preservative
0.09% Sodium azide
Warning
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Preparation and Storage
Store lyophilized product at 2-8 degree C. After reconstitution, aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-Synaptopodin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Synaptopodin antibody
Immunofluorescence, Immunohistochemistry, Western Blot
Application Notes for anti-Synaptopodin antibody
Suitable for use in immunofluorescence, immunohistochemistry (1:5 - 1:10 with frozen sections or paraffin-embedded tissue after microwave treatment), and Western blot

anti-Synaptopodin antibody Western Blot (WB) image
NCBI/Uniprot data below describe general gene information for Synaptopodin. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI Official Full Name
synaptopodin
Protein Family
UniProt Comments for Synaptopodin
SYNPO: an actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.

Protein type: Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: postsynaptic membrane; tight junction; cytoplasm; postsynaptic density; stress fiber; dendritic spine; perikaryon; actin cytoskeleton

Molecular Function: protein binding; actin binding

Biological Process: regulation of stress fiber formation; positive regulation of actin filament bundle formation
Product References and Citations for anti-Synaptopodin antibody
1. Mundel, P., et al., (1991), "Podocytes in Glomerulus of Rat Kidney Express a Characteristic 44 kD Protein", J. Histochem. and Cytochem., Vol. 39, No. 8, 1047-1056.
2. Mundel, P., et al., (1995), "Structure and function of podocytes: an update", Anat. Embryol., 192, 385-397.
3. Mundel, P., et al., (1997), "Phenotypic conversion and differention of human and rat podocytes in vitro", J. Am. Soc. Nephrol., 8, 89708-89705.
4. Mundel, P., et al., (1997), "Synaptopodin, an actin-associated protein in telencephalic dendrites and in renal podocytes", J. Cell Biol., Vol 139(1), 193-204.
5. Mundel, P., et al., (1997), "Rearrangements of cytoskeleton and cell contacts induce process formation and postmitotic differentiation of conditionally immortalized mouse podocyte cell lines", Exp. Cell Res., 236, 248-258.
6. Kobayashi, N., et al., (1998), "Non-uniform microtubular polarity, established by CHO1/MKLP1 motor protein, is necessary for process formation of podocytes", J. Cell Biol., 143, 1961-1970.
7. Barisoni, L., et al., (1999), "The dysregulated podocyte phenotype: a novel concept in the pathogenesis of collapsing idiopathic focal segmental glomerulosclerosis and HIV-associated nephropathy", J. Am. Soc. Nephrol., 10, 51-61.
8. Kihara, I., et al., "Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy", Histopathology, 67(6), 537-547.
Asanuma, K., et al., (2005), "Synaptopodin regulates the actin-binding activity of alpha-actinin in an isoform-specific manner", J. Clin. Invest., 115, 1188-1198

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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