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Synaptopodin Monoclonal Antibody

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Catalog # MBS313029
Unit / Price
  0.05 mg  /  $745 +1 FREE 8GB USB
Western Blot
Product Name

Synaptopodin, Monoclonal Antibody

Popular Item
Full Product Name

MAb to Synaptopodin

Product Synonym Names
Monoclonal Antibody to Synaptopodin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clone Number
Host: Mouse
Source: Cell Culture
Recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of rat brain. Differentiation-dependent expression in cultured hippocampal neurons. Reacts specifically with synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Cross-reacts with human, bovine, rat, mouse, guinea pig and gerbil. Does not cross-react with rabbit, frog or chicken
Protein A chromatography
Purified, Lyophilized. Reconstitute with 1ml distilled water.
50ug/ml (prior to lyophilization) (lot specific)
Isolated rat kidney glomeruli
Affinity Constant
Lyophilized from PBS, pH 7.4 containing 0.5% BSA
0.09% Sodium azide
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Preparation and Storage
Store lyophilized product at 2-8 degree C. After reconstitution, aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-Synaptopodin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Synaptopodin antibody
Immunofluorescence, Immunohistochemistry, Western Blot
Application Notes for anti-Synaptopodin antibody
Suitable for use in immunofluorescence, immunohistochemistry (1:5 - 1:10 with frozen sections or paraffin-embedded tissue after microwave treatment), and Western blot

anti-Synaptopodin antibody Western Blot (WB) image
NCBI/Uniprot data below describe general gene information for Synaptopodin. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI Official Full Name
Protein Family
UniProt Comments for Synaptopodin
SYNPO: an actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.

Protein type: Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: postsynaptic membrane; tight junction; cytoplasm; postsynaptic density; stress fiber; dendritic spine; perikaryon; actin cytoskeleton

Molecular Function: protein binding; actin binding

Biological Process: regulation of stress fiber formation; positive regulation of actin filament bundle formation
Product References and Citations for anti-Synaptopodin antibody
1. Mundel, P., et al., (1991), "Podocytes in Glomerulus of Rat Kidney Express a Characteristic 44 kD Protein", J. Histochem. and Cytochem., Vol. 39, No. 8, 1047-1056.
2. Mundel, P., et al., (1995), "Structure and function of podocytes: an update", Anat. Embryol., 192, 385-397.
3. Mundel, P., et al., (1997), "Phenotypic conversion and differention of human and rat podocytes in vitro", J. Am. Soc. Nephrol., 8, 89708-89705.
4. Mundel, P., et al., (1997), "Synaptopodin, an actin-associated protein in telencephalic dendrites and in renal podocytes", J. Cell Biol., Vol 139(1), 193-204.
5. Mundel, P., et al., (1997), "Rearrangements of cytoskeleton and cell contacts induce process formation and postmitotic differentiation of conditionally immortalized mouse podocyte cell lines", Exp. Cell Res., 236, 248-258.
6. Kobayashi, N., et al., (1998), "Non-uniform microtubular polarity, established by CHO1/MKLP1 motor protein, is necessary for process formation of podocytes", J. Cell Biol., 143, 1961-1970.
7. Barisoni, L., et al., (1999), "The dysregulated podocyte phenotype: a novel concept in the pathogenesis of collapsing idiopathic focal segmental glomerulosclerosis and HIV-associated nephropathy", J. Am. Soc. Nephrol., 10, 51-61.
8. Kihara, I., et al., "Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy", Histopathology, 67(6), 537-547.
Asanuma, K., et al., (2005), "Synaptopodin regulates the actin-binding activity of alpha-actinin in an isoform-specific manner", J. Clin. Invest., 115, 1188-1198

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