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anti-TNNI3 antibody :: Troponin I-Cardiac (27-43 Region) Monoclonal Antibody

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Catalog # MBS311077
Unit / Price
  1 mg  /  $515 +1 FREE 8GB USB
anti-TNNI3 antibody
Product Name

Troponin I-Cardiac (27-43 Region) (TNNI3), Monoclonal Antibody

Popular Item
Also Known As

MAb to Troponin I-Cardiac

Product Synonym Names
Monoclonal Antibody to Troponin I-Cardiac (cTnI)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
210
Chromosome Location
Chromosome: 19; NC_000019.9 (55663135..55669100, complement). Location: 19q13.4
OMIM
115210
3D Structure
ModBase 3D Structure for P19429
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M155
Host
Host: Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Source: Ascites
Specificity
Troponin I-Cardiac (27-43 Region)
Reacts equally with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin, phosphorylation and oxidation. Recognizes an epitope located between amino acid residues 27 and 43 of cTnI. Reactive with cTnI from human, monkey, mouse, rat, canine, rabbit and bovine.
Purity/Purification
>90% pure. Protein A Sepharose chromatography. Purity is tested by electrophoresis.
Form/Format
Purified, Liquid
Concentration
5.3 mg/ml (Sigma protein assay kit) (lot specific)
Immunogen
Free human cardiac troponin and/or native troponin complex
Affinity Constant
Not determined
Buffer
PBS, pH 7.4
Preservative
0.1% Sodium Azide
Warnings
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Preparation and Storage
Store at 2 to 8 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-TNNI3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TNNI3 antibody
EIA/ELISA, LF, Western Blot
Application Notes for anti-TNNI3 antibody
Troponin I immunoassay and troponin I immunoaffinity purification. Works in Western Blotting.
NCBI/Uniprot data below describe general gene information for TNNI3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
24,008 Da[Similar Products]
NCBI Official Full Name
troponin I, cardiac muscle
NCBI Official Synonym Full Names
troponin I type 3 (cardiac)
NCBI Official Symbol
TNNI3  [Similar Products]
NCBI Official Synonym Symbols
CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; MGC116817
  [Similar Products]
NCBI Protein Information
troponin I, cardiac muscle
UniProt Protein Name
Troponin I, cardiac muscle
UniProt Synonym Protein Names
Cardiac troponin I
Protein Family
UniProt Gene Name
TNNI3  [Similar Products]
UniProt Synonym Gene Names
TNNC1  [Similar Products]
UniProt Entry Name
TNNI3_HUMAN
NCBI Summary for TNNI3
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]
UniProt Comments for TNNI3
TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Motor

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: sarcomere; troponin complex; cytosol

Molecular Function: troponin T binding; troponin C binding; protein domain specific binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; protein kinase binding; calcium-dependent protein binding

Biological Process: regulation of smooth muscle contraction; cellular calcium ion homeostasis; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; cardiac muscle contraction; muscle filament sliding

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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