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anti-TNF antibody :: Tumor Necrosis Factor alpha (TNF-a) Monoclonal Antibody

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Catalog # MBS311184
Unit / Price
  1 mg  /  $555 +1 FREE 8GB USB
anti-TNF antibody
Product Name

Tumor Necrosis Factor alpha (TNF-a), Monoclonal Antibody

Also Known As

MAb to TNF Alpha

Product Synonym Names
Monoclonal Antibody to Human Tumor Necrosis Factor alpha (TNF-alpha)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
233
Chromosome Location
Chromosome: 6; NC_000006.11 (31543350..31546112). Location: 6p21.3
OMIM
191160
3D Structure
ModBase 3D Structure for P01375
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2C8
Host
Mouse. Hybridization of X63.Ag8.653 myeloma cells with spleen cells from BALB/c mice.
Specificity
Tumor Necrosis Factor alpha (TNF-a)
Effective in inhibition of the biological activity of recombinant and natural TNF-alpha.
Purity/Purification
>90% pure (SDS-PAGE). Protein A chromatography
Form/Format
Purified, Liquid
Concentration
6.3mg/ml (OD280nm, E^0.1% = 1.4) (lot specific)
Immunogen
Human recombinant tumor necrosis factor of alpha type
Affinity Constant
Not determined
Buffer
PBS, pH 7.4
Preservative
0.1% Sodium azide
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Warnings
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Preparation and Storage
Store at 2 to 8 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-TNF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TNF antibody
EIA/ELISA, Immunohistochemistry - Formalin, Immunoassay Antibody Pairs
Application Notes for anti-TNF antibody
Inhibition of biological activity of TNF-alpha in different biological fluids.
Detection of TNF-alpha in two-site immunoassay.
Immunohistochemistry using formalin-fixed frozen tissues.
Not tested with paraffin-embedded sections.Each laboratory sh
NCBI/Uniprot data below describe general gene information for TNF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,644 Da[Similar Products]
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNF-alpha
  [Similar Products]
NCBI Protein Information
tumor necrosis factor; TNF-a; cachectin; APC1 protein; OTTHUMP00000029281; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2
UniProt Protein Name
Tumor necrosis factor
UniProt Synonym Protein Names
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
TNFA; TNFSF2  [Similar Products]
UniProt Entry Name
TNFA_HUMAN
NCBI Summary for TNF
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq]
UniProt Comments for TNF
Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Subunit structure: Homotrimer.

Subcellular location: Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor, soluble form: Secreted.

Post-translational modification: The soluble form derives from the membrane form by proteolytic processing.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. Ref.18 Ref.19O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. Ref.15

Polymorphism: Genetic variations in TNF influence susceptibility to hepatitis B virus (HBV) infection [

MIM:610424].

Involvement in disease: Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [

MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

Sequence similarities: Belongs to the tumor necrosis factor family.

Sequence caution: The sequence AAF71992.1 differs from that shown. Reason: Frameshift at positions 91 and 157. The sequence CAA75070.1 differs from that shown. Reason: Erroneous gene model prediction.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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