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Mouse West Nile Virus, NS2b/NS3 Protease Monoclonal Antibody

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Catalog # MBS604194
Unit / Price
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  0.1 mg  /  $655 +1 FREE 8GB USB
anti-West Nile Virus, NS2b/NS3 Protease antibody
Product Name

West Nile Virus, NS2b/NS3 Protease, Monoclonal Antibody

Full Product Name

West Nile Virus, NS2b/NS3 Protease (WNV)

Product Synonym Names
Anti -West Nile Virus, NS2b/NS3 Protease (WNV)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG3
Clone Number
8H153
Host
Mouse
Specificity
Recognizes West Nile Virus NS2b subunit.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder in TBS, 5% trehalose. Reconstitution: Reconstitute with sterile TBS. If 1ml of TBS is used, the antibody concentration will be 100ug/ml.
Immunogen
E. coli-derived rwnvNS3/NS2b
Hybridoma
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant West Nile Virus NS3 Protease complex (rwnvNS3/NS2b; aa 1-184 of NS3; Accession # NP_776018, and aa 49-96 of NS2b; Accession # NP_776017).
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-West Nile Virus, NS2b/NS3 Protease antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-West Nile Virus, NS2b/NS3 Protease antibody
NS3 and NS2b are non-structural viral proteins that play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 is a trypsin-like serine protease that requires NS2b as a cofactor.
Applications Tested/Suitable for anti-West Nile Virus, NS2b/NS3 Protease antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Neutralization
Western Blot, Neutralization, Immunoprecipitation, Direct ELISA
Dilution: Western Blot: This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. Using a colorimetric detection system, the detection limit for rwnvNS3/NS2b complex is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Immunoprecipitation: This antibody was used at a concentration of 25ug/mL to immunoprecipitate rwnvNS3/NS2b from conditioned cell culture medium. The recovered rwnvNS3/NS2b can be detected in Western blots using a polyclonal antibody.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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