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anti-CR1 antibody :: Mouse CD35 / CR1 Monoclonal Antibody

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Catalog # MBS438802
Unit / Price
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  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  7 mL (Prediluted)  /  $270 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

CD35 / CR1, Monoclonal Antibody

Popular Item
Full Product Name

CD35 / CR1 (Follicular Dendritic Cell Marker) Mouse Monoclonal Antibody

Product Synonym Names
C3 binding protein, C3b/C4b receptor, C3BR, C4BR, Complement Component (3b/4b) receptor 1 including Knops blood group system, Complement receptor type 1, KN, Knops blood group antigen
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
120620
3D Structure
ModBase 3D Structure for P17927
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
CR1/802
Host
Mouse
Species Reactivity
Human, Baboon, Monkey
Specificity
Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Cellular Localization
Cell Surface
Immunogen
Recombinant human CR1 protein
Hu-Chromosome Location
1q32.2
Positive Control
Follicular dendritic cells (FDC) in tonsil
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-CR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CR1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-CR1 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-CR1 antibody
Formalin-fixed, paraffin-embedded human Tonsil stained with CD35 Monoclonal Antibody (CR1/802).
anti-CR1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CR1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
210-220kDa
NCBI Official Full Name
complement receptor type 1 isoform F
NCBI Official Synonym Full Names
complement component 3b/4b receptor 1 (Knops blood group)
NCBI Official Symbol
NCBI Official Synonym Symbols
KN; C3BR; C4BR; CD35
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NCBI Protein Information
complement receptor type 1
UniProt Protein Name
Complement receptor type 1
UniProt Synonym Protein Names
C3b/C4b receptor; CD_antigen: CD35
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
CR1_HUMAN
NCBI Summary for CR1
This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for CR1
CR1: Mediates cellular binding of particles and immune complexes that have activated complement. Belongs to the receptors of complement activation (RCA) family.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: complement component C3b binding; complement component C3b receptor activity; complement component C4b binding; complement component C4b receptor activity

Biological Process: negative regulation of complement activation, alternative pathway; negative regulation of complement activation, classical pathway; regulation of complement activation

Disease: Knops Blood Group System; Malaria, Susceptibility To
Product References and Citations for anti-CR1 antibody
Ahearn, J.M. and Fearon, D.T. 1989. Structure and function of the complement receptors, CR1 (CD35) and CR2 (CD21). Adv. Immunol. 46: 183-219

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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