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anti-Hsp25/Hsp27 antibody :: Mouse Hsp25/Hsp27 clone 8A7 Monoclonal Antibody

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Catalog # MBS190585
Unit / Price
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  0.1 mg  /  $370 +1 FREE 8GB USB
anti-Hsp25/Hsp27 antibody
Product Name

Hsp25/Hsp27 clone 8A7, Monoclonal Antibody

Full Product Name

Anti-Hsp25/Hsp27 Monoclonal Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clone Number
Species Reactivity
Human, mouse, rat, bovine, canine, guinea pig, hamster
Hsp25/Hsp27 clone 8A7
This antibody recognizes human, mouse, rat, bovine, canine, guinea pig, and hamster Hsp 25/27. Some crossreactivity with alpha B crystallin.
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
Hsp27 peptide
0.09% sodium azide
Dilution Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C.
Other Notes
Small volumes of anti-Hsp25/Hsp27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Hsp25/Hsp27 antibody
Hsp25 is the mouse homologue of human Hsp27, a member of the small heat shock protein family comprised of proteins of ~15->30kDa. Oligomers of Hsp27 consist of as many as 8-40 Hsp27 monomers; large oligomers have high chaperone activity whereas dimers have no chaperone activity. Hsp27 is localized to the cytoplasm of unstressed cells but redistributes to the nucleus in response to stress where it may stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli, and, therefore, has been suggested as an important intermediate in second messengermediated signaling pathways. Hsp27 appears to be involved in cell differentiation and may play a role in termination of cell growth.
Applications Tested/Suitable for anti-Hsp25/Hsp27 antibody
Immunoblot; Immunofluorescence
Application Notes for anti-Hsp25/Hsp27 antibody
Immunoblotting: use at 0.2ug/ml (ECL). A band of 25-27 kDa is detected. Immunofluorescence: use at 5ug/ml. Positive control: HeLa cell lysate
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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