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anti-ACTA1 antibody :: Mouse a-Actin, Skeletal Muscle Monoclonal Antibody

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Catalog # MBS603340
Unit / Price
  0.1 mg  /  $470 +1 FREE 8GB USB
anti-ACTA1 antibody
Product Name

a-Actin, Skeletal Muscle (ACTA1), Monoclonal Antibody

Popular Item
Also Known As

a-Actin, Skeletal Muscle (BSA & Azide Free)

Product Synonym Names
Anti -a-Actin, Skeletal Muscle (BSA & Azide Free)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 3; NC_006090.3 (39337939..39339803, complement). Location: chromosome: 3
3D Structure
ModBase 3D Structure for P68139
Clonality
Monoclonal
Isotype
IgM,k
Clone Number
0.N.4
Host
Mouse
Species Reactivity
Bovine, Frog, Guinea Pig, Human, Rabbit, Rat, Sheep
Specificity
Recognizes human alpha-skeletal and alpha-cardiac (sarcomeric) actins as well as neoplasms derived from normal tissues (i.e. rhabdomyosarcomas). Shows no crossreaction with smooth muscle actin. Species Crossreactivity: bovine, rabbit, sheep, rat, guinea pig, and frog.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See A0760-24.
Immunogen
N-terminal peptide of alpha skeletal muscle actin; acetylated at the N-terminus and conjugated to KLH at the C-terminus. Cellular Localization: Cytoplasmic. Epitope: N-terminal decapeptide.
Positive Control
Skeletal or Cardiac muscle or Rhabdomyosarcoma
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-ACTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ACTA1 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ACTA1 antibody
Suitable for use in Immunofluorescence and Immunohistochemistry.
Dilution: Immunohistochemistry: 2-4ug/ml for 30 min. at RT.
Formalin/paraffin: No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
NCBI/Uniprot data below describe general gene information for ACTA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,051 Da[Similar Products]
NCBI Official Full Name
actin, alpha skeletal muscle
NCBI Official Synonym Full Names
actin, alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1  [Similar Products]
NCBI Protein Information
actin, alpha skeletal muscle; a-actin; alpha-actin-1
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Synonym Protein Names
Alpha-actin-1
UniProt Gene Name
ACTA1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
ACTS_CHICK
UniProt Comments for ACTA1
Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

Subcellular location: Cytoplasm › cytoskeleton.

Post-translational modification: Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization

By similarity.

Miscellaneous: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Sequence similarities: Belongs to the actin family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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