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anti-Cdk9 antibody :: Rabbit cdk9, p43 Monoclonal Antibody

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Catalog # MBS601199
Unit / Price
  0.02 mL  /  $385 +1 FREE 8GB USB
  0.1 mL  /  $540 +1 FREE 8GB USB
anti-Cdk9 antibody
Product Name

cdk9, p43, Monoclonal Antibody

Also Known As

cdk9, p43 (PITALRE, cdc Kinase Family, Cyclin-dependent Kinase 9)

Product Synonym Names
Anti -cdk9, p43 (PITALRE, cdc Kinase Family, Cyclin-dependent Kinase 9)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2R; NT_033778.3 (18523568..18525228, complement). Location: 58F2-58F3
3D Structure
ModBase 3D Structure for O17432
Clonality
Monoclonal
Isotype
IgG
Clone Number
8A9
Host
Rabbit
Species Reactivity
Bovine, Hamster, Human, Monkey, Mouse, Rat
Specificity
Detects endogenous levels of total CDK9 protein, both 42 kDa and 55 kDa isoforms.
Purity/Purification
As reported
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Immunogen
Synthetic peptide (KLH-coupled) corresponding to residues near the carboxy terminus of human CDK9.
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-Cdk9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Cdk9 antibody
P-TEFb is a general transcription factor that regulates transcription elongation through phosphorylation of the C-terminal tail domain (CTD) of RNA polymerase II (RNAP II). The P-TEFb complex composed of a catalytic subunit, CDK9, and its regulatory cyclin partner, which can be cyclin T1, T2a, T2b or K (reviewed in 1,2). P-TEFb is recruited by the HIV Tat protein to allow transcriptional elongation, and subsequent replication of the viral genome. Inhibition of P-TEFb function therefore has potential for HIV therapy. CDK9 exists as two isoforms, an abundant 42kD isoform, and a less abundant 55kD isoform, which contains an amino-terminal extension (3). The two forms likely have distinct purposes based on differential expression during lymphocyte activation (4,5) and on their localization within the nucleus (5). Cyclin dependent kinases (CDKs) are activated in part by cyclin binding and by phosphorylation of a conserved threonine in the T-loop domain. Phosphorylation of CDK9 at the T-loop Thr186 by an unidentified nuclear kinase may be important in P-TEFb activation (6) and regulation of HIV transcription (7). Acetylation of CDK9 at Lys44 affects its ability to phosphorylate the RNAPII CTD (8).
Applications Tested/Suitable for anti-Cdk9 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-Cdk9 antibody
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence and Flow Cytometry
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry (paraffin): 1:200
Immunohistochemistry (frozen): 1:200
Immunofluorescence: 1:25
Flow Cytometry: 1:200
NCBI/Uniprot data below describe general gene information for Cdk9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
46,787 Da[Similar Products]
NCBI Official Full Name
Cyclin-dependent kinase 9
NCBI Official Synonym Full Names
Cyclin-dependent kinase 9
NCBI Official Symbol
NCBI Official Synonym Symbols
cdk9; CDK9; CG5179; DmelCG5179; P-TEF; P-TEFb; PTefb
  [Similar Products]
NCBI Protein Information
CG5179 gene product from transcript CG5179-RA; Cyclin-dependent kinase 9; CG5179-PA; Cdk9-PA; positive transcription elongation factor b, small subunit; Cyclin-dependent kinase9 2.7; p43 PITALRE
UniProt Protein Name
Cyclin-dependent kinase 9
Protein Family
UniProt Gene Name
UniProt Entry Name
O17432_DROME
UniProt Comments for Cdk9
Sequence similarities: Belongs to the protein kinase superfamily. RuleBase RU000305V3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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