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anti-CCNA2 antibody :: Mouse cyclin A Monoclonal Antibody

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Catalog # MBS440093
Unit / Price
  0.1 mg  /  $340 +1 FREE 8GB USB
anti-CCNA2 antibody
Product Name

cyclin A (CCNA2), Monoclonal Antibody

Also Known As

cyclin A (E23.1) Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
432
OMIM
123835
3D Structure
ModBase 3D Structure for P20248
Clonality
Monoclonal
Host
Mouse
Species Reactivity
Mouse, bovine, human
Specificity
Reacts with cyclin A of murine, bovine, and human origin
Form/Format
100 ug/ml of mouse monoclonal IgG2a in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Concentration
0.100 mg/ml (lot specific)
Origin
Cyclin A (E67.1) is provided as mouse monoclonal IgG2a derived from a mouse immunized with bovine cyclin A. The epitope for this antibody has been mapped to a region containing the sequence LDYP. This epitope maps within amino acids 144-148 of human cyclin A2.Cyclin As that contain this epitope: Cow cyclin A2, human cyclin A2, hamster cyclin A2, mouse cylin A2.Cyclin As that do not contain this epitope: Chicken cyclin A2, human cyclin A1, mouse cyclin A1
Immunogen
Bovine cyclin A
Preparation and Storage
Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
Other Notes
Small volumes of anti-CCNA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CCNA2 antibody
During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Cyclin A regulates at least two cdks, Cdc2 p34 and Cdk2, and is believed to be necessary for progression through S phase and is active during the G2-M transition (1&3,4). Cyclin B1 also associates with Cdc2 p34 and this complex is requisite for the G2-M progression. The Cyclin B1/Cdc2 p34 complex is known as MPF, M-phase kinase, maturation-promoting factor, or M-phase specific histone kinase, is widely accepted as the trigger of mitosis in all organisms (5-9).
Applications Tested/Suitable for anti-CCNA2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-CCNA2 antibody
Western blotting starting dilution: 1:100.
NCBI/Uniprot data below describe general gene information for CCNA2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
48,551 Da[Similar Products]
NCBI Official Full Name
cyclin-A2
NCBI Official Synonym Full Names
cyclin A2
NCBI Official Symbol
CCNA2  [Similar Products]
NCBI Official Synonym Symbols
CCN1; CCNA
  [Similar Products]
NCBI Protein Information
cyclin-A2
UniProt Protein Name
Cyclin-A2
Protein Family
UniProt Gene Name
CCNA2  [Similar Products]
UniProt Synonym Gene Names
CCN1; CCNA; Cyclin-A  [Similar Products]
UniProt Entry Name
CCNA2_HUMAN
NCBI Summary for CCNA2
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq, Jul 2008]
UniProt Comments for CCNA2
CCNA2: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: nucleoplasm; male pronucleus; cytoplasm; female pronucleus; nucleus

Molecular Function: protein binding; protein kinase binding

Biological Process: mitosis; organ regeneration; response to glucagon stimulus; positive regulation of transcription, DNA-dependent; response to estradiol stimulus; positive regulation of fibroblast proliferation; cell division; mitotic cell cycle G2/M transition DNA damage checkpoint; regulation of G2/M transition of mitotic cell cycle; Ras protein signal transduction; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle
Product References and Citations for anti-CCNA2 antibody
1. Bandara LR, Adamczewski JP, Zamanian M, Poon RY, Hunt T, Thangue NB. 1992. Cyclin A recruits p33cdk2 to the cellular transcription factor DRTF1. J Cell Sci Suppl 16: 77-85.2. Desdouets C, Sobczak-Thepot J, Murphy M, Brechot C. 1995. Cyclin A: Function and expression during cell proliferation. Prog Cell Cycle Res 1:115-123.3. Brechot C. 1993. Oncogenic activation of cyclin A. Curr Opin Genet Dev 3(1): 11-18.4. Goldstone S, Pavey S, Forrest A, Sinnamon J, Gabrielli B. 2001. Cdc25-dependent activation of cyclin A/cdk2 is blocked in G2 phase arrested cells independently of ATM/ATR. Oncogene 20(8): 921-932.5. Liu D, Liao C, Wolgemuth DJ. 2000. A role for cyclin A1 in the activation of MPF and G2-M transition during meiosis of male germ cells in mice. Dev Biol 224(2): 338-400.6. Hoffman I, Clarke PR, Marcotte MJ, Karsenti E, Draetta G. 1993. Phosphorylation and activation of human cdc25-C by cdc2-cyclin B and its involvement in the self-amplification of MPF at mitosis. EMBO J 12(1): 53-63. 7. Kishimoto T, Okumura E. 1997. In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. Prog Cell Cycle Res 3:241-249.8. Baratte B, Meijer L, Galaktionov K, Beach D. 1992. Screening for antimitotic compounds using the cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2/cyclin Bcdc13 protein kinase. Anticancer Res 12(3): 873-880.9. Thron CD. 1994. Theoretical dynamics of the cyclin B-MPF system: a possible role for p13suc1. Biosystems 32(2):97-109.

Precautions
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Disclaimer
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