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anti-HMOX1 antibody :: Mouse HO-1 Monoclonal Antibody

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Catalog # MBS801987
Unit / Price
  0.1 mg  /  $440 +1 FREE 8GB USB
Product Name

HO-1 (HMOX1), Monoclonal Antibody

Also Known As

HO-1 Antibody: PerCP

Product Synonym Names
Hsp32; HMOX1; Heme oxygenase 1; HO; HO1
Matching Pairs
Unconjugated Antibody: HO-1 Clone #1F12-A6 (MBS800054)
PerCP Conjugated Antibody: HO-1 Clone #1F12-A6 (MBS801987)
Matching Pairs
Unconjugated Antibody: HO-1 Clone #1F12-A6 (MBS800757)
PerCP Conjugated Antibody: HO-1 Clone #1F12-A6 (MBS801987)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
288
OMIM
gene 614034
3D Structure
ModBase 3D Structure for P09601
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
1F12-A6
Host
Mouse
Species Reactivity
Human, Mouse, Bovine
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Conjugate
PerCP
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Other Notes
Small volumes of anti-HMOX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HMOX1 antibody
Background Info: Detects a 32kDa protein, corresponding to the molecular mass of HO-1 on SDS Page immunoblots. Does not cross-react with HO-2

Scientific Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
Applications Tested/Suitable for anti-HMOX1 antibody
Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-HMOX1 antibody
1:500-1000 (WB)

Immunohistochemistry (IHC) of anti-HMOX1 antibody
Immunohistochemistry analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: muscle, dermis, hair follicles, epidermis: nuclear everywhere and some cytoplasmic staining.
anti-HMOX1 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-HMOX1 antibody
Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
anti-HMOX1 antibody Western Blot (WB) (WB) image
Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-HMOX1 antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1 Monoclonal Antibody, Clone 1F12-A6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cell-cell border staining in epidermis, punctuate nuclear staining.
anti-HMOX1 antibody Immunocytochemistry/Immunofluorescence (ICC/IF) (ICC) image
NCBI/Uniprot data below describe general gene information for HMOX1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
32,819 Da[Similar Products]
NCBI Official Full Name
heme oxygenase 1
NCBI Official Synonym Full Names
heme oxygenase (decycling) 1
NCBI Official Symbol
HMOX1  [Similar Products]
NCBI Official Synonym Symbols
HO-1; HSP32; HMOX1D; bK286B10
  [Similar Products]
NCBI Protein Information
heme oxygenase 1; heat shock protein, 32-kD
UniProt Protein Name
Heme oxygenase 1
UniProt Gene Name
HMOX1  [Similar Products]
UniProt Synonym Gene Names
HO; HO1; HO-1  [Similar Products]
UniProt Entry Name
HMOX1_HUMAN
NCBI Summary for HMOX1
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]
UniProt Comments for HMOX1
HMOX1: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Expressed at higher levels in renal cancer tissue than in normal tissue. Belongs to the heme oxygenase family.

Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular space; endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; nucleolus; caveola; cytosol; nucleus

Molecular Function: signal transducer activity; protein binding; protein homodimerization activity; enzyme binding; metal ion binding; phospholipase D activity; heme binding; heme oxygenase (decyclizing) activity

Biological Process: response to nicotine; cell death; cellular iron ion homeostasis; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; negative regulation of mast cell degranulation; positive regulation of vasodilation; excretion; erythrocyte homeostasis; regulation of transcription factor activity; heme catabolic process; regulation of blood pressure; small GTPase mediated signal transduction; porphyrin metabolic process; negative regulation of mast cell cytokine production; angiogenesis; negative regulation of neuron apoptosis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; transmembrane transport; healing during inflammatory response; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription factor activity; heme oxidation; cellular response to nutrient; regulation of angiogenesis; negative regulation of leukocyte migration; negative regulation of DNA binding; positive regulation of angiogenesis; iron ion homeostasis; positive regulation of chemokine biosynthetic process; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; response to estrogen stimulus; endothelial cell proliferation; response to oxidative stress; smooth muscle hyperplasia

Disease: Heme Oxygenase 1 Deficiency; Pulmonary Disease, Chronic Obstructive
Product References and Citations for anti-HMOX1 antibody
1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411-419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925-10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759-1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129-135.
1. Al-Rifai, N., Rcker, H., Amslinger, S. (2013). Opening or Closing the Lock? When Reactivity Is the Key to Biological Activity. Chemistry - A Euro J. Article first published online: Sept 17, 2013. DOI: 10.1002/chem.201302117

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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