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anti-HSPG2 antibody :: Rat Heparan Sulfate Proteoglycan, Domain 4 Monoclonal Antibody

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Catalog # MBS600265
Unit / Price
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  0.5 mL  /  $665 +1 FREE 8GB USB
anti-HSPG2 antibody
Product Name

Heparan Sulfate Proteoglycan, Domain 4 (HSPG2), Monoclonal Antibody

Full Product Name

Heparan Sulfate Proteoglycan, Domain 4 (Perlecan, HSPG)

Product Synonym Names
Anti -Heparan Sulfate Proteoglycan, Domain 4 (Perlecan, HSPG)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (22148737..22263750, complement). Location: 1p36.1-p34
OMIM
142461
3D Structure
ModBase 3D Structure for P98160
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
0.N.325 (A7L6)
Host
Rat
Species Reactivity
Bovine, Human, Mouse, Porcine
Specificity
Recognizes mouse Heparan Sulfate Proteoglycan. Does not crossreact with laminin, fibronectin or dermatran sulfate proteoglycan. Species crossreactivity: Human, bovine, porcine
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See H1890-92X.
Immunogen
Mouse EHS laminin preparation. Cellular Localization: Basement membrane
Positive Control
Tonsil
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-HSPG2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HSPG2 antibody
Perlecan is a major heparan sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth facors, perlecan hs been stronglyimplicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor. Suppression of perlican causes substantial inhibition of neoplastic growth and neovasacularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogaenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
Applications Tested/Suitable for anti-HSPG2 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-HSPG2 antibody
Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot.
Dilution: Immunohistochemistry (Frozen and acid-alcohol fixed, paraffin embedded sections): 1-2ug/ml for 30 minutes at RT. Staining of acid-alcohol-fixed tissues requires antigen unmasking with 15,000u/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37 degree C
NCBI/Uniprot data below describe general gene information for HSPG2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
468,830 Da[Similar Products]
NCBI Official Full Name
heparan sulfate proteoglycan perlecan
NCBI Official Synonym Full Names
heparan sulfate proteoglycan 2
NCBI Official Symbol
HSPG2  [Similar Products]
NCBI Official Synonym Symbols
PLC; SJA; SJS; HSPG; SJS1; PRCAN
  [Similar Products]
NCBI Protein Information
basement membrane-specific heparan sulfate proteoglycan core protein; perlecan proteoglycan; endorepellin (domain V region)
UniProt Protein Name
Basement membrane-specific heparan sulfate proteoglycan core protein
UniProt Synonym Protein Names
Perlecan
Protein Family
UniProt Gene Name
HSPG2  [Similar Products]
UniProt Synonym Gene Names
HSPG; PLC  [Similar Products]
UniProt Entry Name
PGBM_HUMAN
NCBI Summary for HSPG2
This gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and Transthyretin, etc. and plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and Tardive dyskinesia.[provided by RefSeq, Mar 2010]
UniProt Comments for HSPG2
HSPG2: Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development. Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1); a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses. Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH). The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.

Protein type: Cell adhesion; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 1p36.1-p34

Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; focal adhesion; Golgi lumen; plasma membrane; extracellular region; basal lamina

Molecular Function: protein C-terminus binding; protein binding; metal ion binding

Biological Process: cardiac muscle development; phototransduction, visible light; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; lipoprotein metabolic process; pathogenesis; embryonic skeletal morphogenesis; chondroitin sulfate metabolic process; extracellular matrix disassembly; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; protein localization; carbohydrate metabolic process; chondrocyte differentiation; angiogenesis; brain development; retinoid metabolic process; endochondral ossification

Disease: Schwartz-jampel Syndrome, Type 1; Dyssegmental Dysplasia, Silverman-handmaker Type
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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