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anti-Cdkn1a antibody :: Mouse p21,WAF1 Monoclonal Antibody

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Catalog # MBS603733
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anti-Cdkn1a antibody
Product Name

p21,WAF1 (Cdkn1a), Monoclonal Antibody

Full Product Name

p21,WAF1 (Cip1, Sdi1, Pic1,Wild-type p53-activated Fragment, Tumor Suppressor Protein)

Product Synonym Names
Anti -p21,WAF1 (Cip1, Sdi1, Pic1,Wild-type p53-activated Fragment, Tumor Suppressor Protein)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 17; NC_000083.6 (29090986..29100722). Location: 17 A3.3; 17 15.12 cM
3D Structure
ModBase 3D Structure for P39689
Clonality
Monoclonal
Isotype
IgG1
Clone Number
0.N.489
Host
Mouse
Species Reactivity
Bovine, Human, Mouse, Rat
Specificity
Recognizes Human, bovine, Mouse and Rat p21WAF1/Cip1/Sdi1/Pic1 tumor suppressor protein.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide. Also supplied without BSA and sodium azide. See P1000-55X.
Immunogen
Full length human recombinant p21 protein.
Cellular Localization
Nuclear
Hybridoma
Produced by fusion between RBF/DnJ splenocytes and mouse myeloma NS1 cells.
Positive Control
Ls174T or Raji cells. Colon CA.
Preparation and Storage
-20 degree C
Other Notes
Small volumes of anti-Cdkn1a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Cdkn1a antibody
Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. Meanwhile, the p21WAF1 protein also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process. Evidently, inactivation of p53 disrupts the p53-dependent expression of p21WAF1. p21WAF1is a tumor suppressor involved in the pathogenesis of a variety of malignancies.
Applications Tested/Suitable for anti-Cdkn1a antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
NCBI/Uniprot data below describe general gene information for Cdkn1a. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
17,785 Da[Similar Products]
NCBI Official Full Name
p21
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 1A (P21)
NCBI Official Symbol
Cdkn1a  [Similar Products]
NCBI Official Synonym Symbols
P21; CDKI; CIP1; SDI1; Waf1; mda6; CAP20; Cdkn1; p21WAF; p21Cip1
  [Similar Products]
NCBI Protein Information
cyclin-dependent kinase inhibitor 1; p21<WAF1/CIP1>; CDK-interacting protein 1; melanoma differentiation-associated protein
UniProt Protein Name
Cyclin-dependent kinase inhibitor 1
UniProt Synonym Protein Names
CDK-interacting protein 1; Melanoma differentiation-associated protein; p21
UniProt Gene Name
Cdkn1a  [Similar Products]
UniProt Synonym Gene Names
Cip1; Waf1  [Similar Products]
UniProt Entry Name
CDN1A_MOUSE
UniProt Comments for Cdkn1a
p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase.

Protein type: Inhibitor; Cell cycle regulation

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; intracellular; cytosol; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; cyclin binding; cyclin-dependent protein kinase activating kinase activity; protein kinase inhibitor activity; ubiquitin protein ligase binding; metal ion binding; protein complex binding

Biological Process: regulation of cell cycle; regulation of mitotic cell cycle; negative regulation of cyclin-dependent protein kinase activity; cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; protein amino acid phosphorylation; negative regulation of cell proliferation; positive regulation of programmed cell death; positive regulation of fibroblast proliferation; positive regulation of protein kinase activity; positive regulation of B cell proliferation; cellular response to extracellular stimulus; negative regulation of phosphorylation; regulation of protein import into nucleus, translocation; negative regulation of cell growth; regulation of cyclin-dependent protein kinase activity; cell cycle arrest; G2/M transition of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to DNA damage stimulus; G1/S transition of mitotic cell cycle; negative regulation of apoptosis; response to UV
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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