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anti-LGR5 antibody :: Mouse LGR5/GPR49 Monoclonal Antibody

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Catalog # MBS9200598
Unit / Price
  0.1 mL  /  $340 +1 FREE 8GB USB
Western Blot (WB)
Product Name

LGR5/GPR49, Monoclonal Antibody

Popular Item
Also Known As

LGR5/GPR49 Antibody (Ascites)

Product Synonym Names
Leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; LGR5; GPR49; GPR67
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
443-473
Immunogen Sequence Length
883
OMIM
606667
3D Structure
ModBase 3D Structure for O75473
Clonality
Monoclonal
Isotype
IgG1
Clone Number
349CT16.1.3.1
Host
Mouse
Species Reactivity
Human (Predicted Reactivity: Bovine)
Specificity
This LGR5/GPR49 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 443-473 amino acids from human LGR5/GPR49.
Form/Format
Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Function
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Cellular Location
Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653).
Tissue Location
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base.Overexpressed in cancers of the ovary, colon and liver.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-LGR5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-LGR5 antibody
Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
Applications Tested/Suitable for anti-LGR5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-LGR5 antibody
WB~~1:1000~8000

Western Blot (WB) of anti-LGR5 antibody
LGR5/GPR49 Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).
anti-LGR5 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for LGR5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Human (Predicted Reactivity: Bovine)NP_003658.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
99998
NCBI Official Full Name
leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2
NCBI Official Synonym Full Names
leucine-rich repeat containing G protein-coupled receptor 5
NCBI Official Symbol
NCBI Official Synonym Symbols
FEX; HG38; GPR49; GPR67; GRP49
  [Similar Products]
NCBI Protein Information
leucine-rich repeat-containing G-protein coupled receptor 5
UniProt Protein Name
Leucine-rich repeat-containing G-protein coupled receptor 5
UniProt Synonym Protein Names
G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38
UniProt Gene Name
UniProt Synonym Gene Names
GPR49; GPR67  [Similar Products]
UniProt Entry Name
LGR5_HUMAN
NCBI Summary for LGR5
The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
UniProt Comments for LGR5
LGR5: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A marker of cycling, long-lived and multipotent adult stem cell populations. Target gene of Wnt signaling. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Intestinal Lgr5+ stem cells give rise throughout life to proliferation of cells in crypts, which migrate along the crypt-villus axis, differentiating into five main cell types. Wnt signaling plays a crucial role in the proliferation and differentiation of these crypt epithelial cells. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: integral to plasma membrane; plasma membrane; trans-Golgi network membrane

Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; inner ear development
Product References and Citations for anti-LGR5 antibody
Fontaine-Bisson, B., et al. Diabetologia 53(10):2155-2162(2010)
Voight, B.F., et al. Nat. Genet. 42(7):579-589(2010)
Uchida, H., et al. Cancer Sci. 101(7):1731-1737(2010)
Fan, X.S., et al. Int J Colorectal Dis 25(5):583-590(2010)
Acevedo, A.C., et al. J. Dent. Res. 89(2):128-132(2010)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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