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anti-CD20 antibody :: Mouse CD20 Monoclonal Antibody

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Catalog # MBS370021
Unit / Price
  5 Control Slides  /  $130 +1 FREE 8GB USB
  3 ml (Prediluted)  /  $165 +1 FREE 8GB USB
  0.1 mL (Concentrate)  /  $180 +1 FREE 8GB USB
  7 mL (Prediluted)  /  $190 +1 FREE 8GB USB
  0.5 mL (Concentrate)  /  $245 +1 FREE 8GB USB
  15 mL (Prediluted)  /  $295 +1 FREE 8GB USB
  1 mL (Concentrate)  /  $300 +1 FREE 8GB USB
Product Name

CD20, Monoclonal Antibody

Popular Item
Full Product Name

CD20

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 21; NC_006603.2 (53903583..53913185)
3D Structure
ModBase 3D Structure for Q3C2E2
Clonality
Monoclonal
Isotype
IgG2a/K
Clone Number
L26
Host
Mouse
Species Reactivity
Human, Canine, Feline, Mouse
Form/Format
CD20 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Immunogen
Human Tonsil B cells
Control
Tonsil, Lymph Node
Localization
Membranous
Reactivity Note
Paraffin, Frozen
Specimen Preparation
Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.
Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
Staining Procedure
1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA
5. Any of three heating methods may be used:
a. TintoRetriever Pressure Cooker or Equivalent
Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. TintoRetriever PT Module or Water Bath Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.
c. Conventional Steamer Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.
8. Wash slides with IHC wash buffer or DI water.
9. Continue IHC staining protocol.
Product Limitations
Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls. Results should be interpreted by a medical professional.
Note
This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up.
Preparation and Storage
Store at 2 to 8 degree C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.
Other Notes
Small volumes of anti-CD20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD20 antibody
CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B-cells, but lost following differentiation into plasma cells. This antibody does not cross-react with non-hematopoietic neoplasms. CD20 (B-cell Pan) reacts with a membrane antigen present in B-cells. This antibody strongly recognizes Reed-Sternberg cells predominant in Hodgkin's disease. Since no staining of histiocytes or plasma cells has been observed and CD20 has not been detected in T-cell malignancies, it is a very strong marker of B-cell Lymphomas. B-cell Panmarker recognizes a formalin-resistant intracytoplasmic antigen.
Application Notes for anti-CD20 antibody
Intended Use: This antibody is intended for use in Immunohistochemical applications on formalin fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretations of results should be performed by a qualified medical professional.

Dilution Information of anti-CD20 antibody
anti-CD20 antibody Dilution Information image
Recommended IHC Protocol of anti-CD20 antibody
anti-CD20 antibody Recommended IHC Protocol image
Performance Characteristics of anti-CD20 antibody
anti-CD20 antibody Performance Characteristics image
NCBI/Uniprot data below describe general gene information for CD20. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
33,056 Da
NCBI Official Full Name
CD20
NCBI Official Symbol
MS4A1  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
B-lymphocyte antigen CD20; membrane-spanning 4-domains subfamily A member 1
UniProt Protein Name
B-lymphocyte antigen CD20
UniProt Synonym Protein Names
Membrane-spanning 4-domains subfamily A member 1
Protein Family
UniProt Gene Name
MS4A1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
CD20_CANFA
UniProt Comments for CD20
Function: This protein may be involved in the regulation of B-cell activation and proliferation

Subcellular location: Membrane; Multi-pass membrane protein

Tissue specificity: Expressed in PBMCs and lymph node from healthy dogs, in B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells. Ref.1

Post-translational modification: Phosphorylated. Might be functionally regulated by protein kinase(s)

Sequence similarities: Belongs to the MS4A family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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