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Mouse a-Dystroglycan Monoclonal Antibody

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Catalog # MBS600655
Unit / Price
  0.2 mL  /  $655 +1 FREE 8GB USB
anti-a-Dystroglycan antibody
Product Name

a-Dystroglycan, Monoclonal Antibody

Popular Item
Full Product Name

a-Dystroglycan (Cranin)

Product Synonym Names
Anti -a-Dystroglycan (Cranin)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgM
Clone Number
4A145
Host
Mouse
Species Reactivity
Canine, Guinea Pig, Human, Mouse, Rabbit, Rat
Specificity
Recognizes rabbit alpha-Dystroglycan at ~156kD. Species Crossreactivity: human, mouse, rat, canine and guinea pig.
Purity/Purification
Ascites
Ascites
Form/Format
Supplied as a liquid in PBS, 0.05% sodium azide, 30% glycerol.
Immunogen
Rabbit skeletal muscle membrane preparation.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-a-Dystroglycan antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-a-Dystroglycan antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-a-Dystroglycan antibody
Suitable for use in ELISA, Inhibition of Laminin Binding to Dystroglycan and Western Blot.
Dilution: Inhibition of Laminin Binding to Dystroglycan: In a nitrocellulose overlay experiment, this antibody inhibits binding of 125I Laminin to Dystroglycan.
Western Blot: 1:1000-1:2000 Detects alpha-Dystroglycan in rabbit skeletal muscle. Rabbit skeletal muscle lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with D9905-05. Proteins were visualized using an IgM (HRP) goat anti-mouse and a chemiluminescence detection system.
Note: The use of WGA puri ed protein results in signi cantly cleaner blots and immunoprecipitates. Post-translational modi cation of Dystroglycan causes band broadening.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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