Name: anti-a-Dystroglycan antibody; Mouse a-Dystroglycan Monoclonal Antibody (Clone 4A145)
Brand: MyBioSource
Price: 615 USD

Product Name

a-Dystroglycan, Monoclonal Antibody

a-Dystroglycan (Cranin)

Product Synonym Names

Anti -a-Dystroglycan (Cranin)

Research Use Only

For Research Use Only. Not for use in diagnostic procedures.

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Clonality

Monoclonal

Isotype

IgM

Clone Number

4A145

Host

Mouse

Species Reactivity

Canine, Guinea Pig, Human, Mouse, Rabbit, Rat

Specificity

Recognizes rabbit alpha-Dystroglycan at ~156kD. Species Crossreactivity: human, mouse, rat, canine and guinea pig.

Purity/Purification

Ascites
Ascites

Form/Format

Supplied as a liquid in PBS, 0.05% sodium azide, 30% glycerol.

Immunogen

Rabbit skeletal muscle membrane preparation.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Other Notes

Small volumes of anti-a-Dystroglycan antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

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Product Categories/Family for anti-a-Dystroglycan antibody

Antibodies; Abs to Carbohydrates, Glycoproteins

Applications Tested/Suitable for anti-a-Dystroglycan antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-a-Dystroglycan antibody

Suitable for use in ELISA, Inhibition of Laminin Binding to Dystroglycan and Western Blot.
Dilution: Inhibition of Laminin Binding to Dystroglycan: In a nitrocellulose overlay experiment, this antibody inhibits binding of 125I Laminin to Dystroglycan.
Western Blot: 1:1000-1:2000 Detects alpha-Dystroglycan in rabbit skeletal muscle. Rabbit skeletal muscle lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with D9905-05. Proteins were visualized using an IgM (HRP) goat anti-mouse and a chemiluminescence detection system.
Note: The use of WGA puri ed protein results in signi cantly cleaner blots and immunoprecipitates. Post-translational modi cation of Dystroglycan causes band broadening.

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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Catalog #	MBS600655
Unit / Price	0.2 mL / $615 +1 FREE 8GB USB