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anti-SHC1 antibody :: Mouse Shc Monoclonal Antibody

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Catalog # MBS603009
Unit / Price
  0.1 mg  /  $795 +1 FREE 8GB USB
anti-SHC1 antibody
Product Name

Shc (SHC1), Monoclonal Antibody

Popular Item
Also Known As

Shc, phosphorylated (Tyr317) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Product Synonym Names
Anti -Shc, phosphorylated (Tyr317) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (154934774..154946959, complement). Location: 1q21
OMIM
600560
3D Structure
ModBase 3D Structure for P29353
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6D758
Host
Mouse
Species Reactivity
Canine, Human, Mouse
Specificity
Recognizes human shc phosphorylated at Tyr317. Species Crossreactivity: mouse and canine.
Purity/Purification
Purified
Purified by subsequent thiophillic adsorption and size exclusion chromatography.
Form/Format
Supplied as a liquid in PBS, 0.1% sodium azide, PEG, sucrose, before the addition of glycerol to 40%
Concentration
0.1 mg/ml (lot specific)
Immunogen
Synthetic peptide corresponding to aa 314-320 (DPSpYVNV) of human shc phosphorylated at Tyr317 (KLH).
Positive Control Included
HepG2 Cell Lysate, Vanadate Treated. Add 100ul SDS-PAGE sample buffer and incubate at 90 Deg. C for 5 minutes. Aliquot and store at - 20 Degree C.
Western Blot: 20ul/lane (mini gel) for HRP/ECL detection.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-SHC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SHC1 antibody
The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p66 is generated form a distinct mRNA. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type. Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span.
Applications Tested/Suitable for anti-SHC1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-SHC1 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1ug/ml for HRPO/ECL detection. Use Casein/Tween-20 based blocking and Blot incubation buffers.
ELISA: 0.05ug/ml.
Optimal conditions must be determined individually for each application.
NCBI/Uniprot data below describe general gene information for SHC1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
62,822 Da[Similar Products]
NCBI Official Full Name
SHC-transforming protein 1 isoform 2
NCBI Official Synonym Full Names
SHC (Src homology 2 domain containing) transforming protein 1
NCBI Official Symbol
NCBI Official Synonym Symbols
SHC; SHCA
  [Similar Products]
NCBI Protein Information
SHC-transforming protein 1; SH2 domain protein C1; SHC-transforming protein 3; SHC-transforming protein A; SHC (Src homology 2 domain-containing) transforming protein 1
UniProt Protein Name
SHC-transforming protein 1
UniProt Synonym Protein Names
SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
SHC; SHCA; SH2 domain protein C1  [Similar Products]
UniProt Entry Name
SHC1_HUMAN
NCBI Summary for SHC1
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
UniProt Comments for SHC1
SHC1: an adaptor protein containing a SH2 domain and a PID domain within a PH domain-like fold. Couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Six human isoforms are produced by alternative promoter usage and alternative splicing. Isoforms p66, p52 and p46 (P29353-1, -2, and -3), produced by alternative initiation, variously regulate growth factor signaling, oncogenesis, intracellular oxidant levels, and apoptosis. Isoforms p46 and p52, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex, thus initiating the cytoplasmic proliferative Ras signaling cascade in various non-neuronal systems. Isoform p66 does not mediate Ras activation, but associates with mitochondria where it controls intracellular redox status, mitochondrial permeability, life span, and stress-induced apoptosis. p66 acts as a downstream target of the tumor suppressor p53 and is required for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. P66 deletion in mice decreases the incidence of aging-associated diseases, such as atherosclerosis, and significantly prolongs life span. Participates in signaling downstream of TIE2, the tyrosine kinase receptor for angiopoietin, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. Interacts with tyrosine-phosphorylated CD3T, DDR2, LRP1, IRS4, SHP, FLT4, PDGFRB, TIE2, TrkA, -B and -C. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR in vitro via the PID domain. p66Shc is known to be activated by the mutant SOD1 associated with familial forms of amyotrophic lateral sclerosis (ALS), causing a decrease in the activity of Rac1 through a redox-sensitive regulation. In case of oxidative conditions, phosphorylation at S36 of isoform p66Shc, leads to mitochondrial accumulation p66 plays a role in mediating mitophagy and determining neuronal cell fate following acute oxygen glucose deprivation. Isoform p46 is localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.

Protein type: Apoptosis; Motility/polarity/chemotaxis; Mitochondrial; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: mitochondrial matrix; plasma membrane; cytosol

Molecular Function: insulin-like growth factor receptor binding; protein binding; ephrin receptor binding; protein-tyrosine kinase activity; neurotrophin TRKA receptor binding; phospholipid binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; protein kinase binding; insulin receptor binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; platelet activation; peptidyl-tyrosine phosphorylation; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; unfolded protein response; activation of MAPK activity; heart development; regulation of epidermal growth factor receptor activity; MAPKKK cascade; cell-cell adhesion; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of growth; actin cytoskeleton reorganization; positive regulation of cell proliferation; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; angiogenesis; blood coagulation; leukocyte migration; positive regulation of DNA replication
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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