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anti-CD21 antibody :: Mouse CD21 Monoclonal Antibody

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Catalog # MBS215219
Unit / Price
  100 Tests  /  $660 +1 FREE 8GB USB
Product Name

CD21, Monoclonal Antibody

Popular Item
Also Known As

MOUSE ANTI CANINE CD21:RPE

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CA2.1D6
Host
Mouse
Species Reactivity
Horse, Cat, Racoon
N.B. Antibody reactivity and working conditions vary between species
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Max Ex/Em
Fluorophore
PE 488nm laser
PE 561nm laser
Excitation (nm):
496
546
Emission (nm):
578
578
Buffer Solution
Phosphate buffered saline
Target Species
Dog
Preparation and Storage
Prior to reconstitution store at 4°C. Following reconstitution store at 4°C.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Other Notes
Small volumes of anti-CD21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD21 antibody
Mouse anti Canine CD21 antibody, clone CA2.1D6 recognizes canine CD21, also known as Complement receptor type 2. CD21is a cell surface antigen expressed by canine B lymphocytes.
The antigen recognised may be the canine homologue of human CD21, but this has not been fully confirmed.
Mouse anti Canine CD21 antibody, clone CA2.1D6 also recognizes the CD21 antigen in Felids. Expression in cats is analogous to that seen in dogs with strong expression on lymphocytes, in a manner mutually exclusive with expression of CD4 or CD8. Clone CA2.1D6 also immunoprecipitates a 145kDa protein from feline lymphocytes, similar to the protein immunoprecipitated by the antibody from canine lymphocytes (Dean et al. 1996).
Applications Tested/Suitable for anti-CD21 antibody
Flow cytometry
Application Notes for anti-CD21 antibody
Dilution: Neat
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
Note:N.B. It should NOT be used with (mouse anti canine CD3), in dual colour flow cytometry, due to non-specific interactions between the two reagents.

Testing Data of anti-CD21 antibody
Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21:RPE
anti-CD21 antibody Testing Data image
Testing Data of anti-CD21 antibody
Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21:Alexa Fluor 647
anti-CD21 antibody Testing Data image
Testing Data of anti-CD21 antibody
Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21 (MBS215220)
anti-CD21 antibody Testing Data image
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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