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anti-PCNA antibody :: Mouse PCNA (Proliferating Cell Nuclear Antigen) Monoclonal Antibody

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Catalog # MBS438057
Unit / Price
  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  7 ml (Prediluted)  /  $270 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

PCNA (Proliferating Cell Nuclear Antigen), Monoclonal Antibody

Popular Item
Also Known As

PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Mouse Monoclonal Antibody

Product Synonym Names
Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
261
OMIM
176740
3D Structure
ModBase 3D Structure for P12004
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
SPM350
Host
Mouse
Species Reactivity
Human, Monkey, Pig, Mouse, Rat, Chicken, Zebrafish, Drosophila melanogaster, Yeast (S. pombe and S. cerevisiae)
Specificity
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Cellular Localization
Predominantly nuclear, some cytoplasmic
Immunogen
Rat PCNA/Protein A fusion protein
Hu-Chromosome Location
20p12.3
Positive Control
Tonsil or reactive lymph node
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-PCNA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PCNA antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-PCNA antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-PCNA antibody
Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Monoclonal Antibody (SPM350)
anti-PCNA antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PCNA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
36kDa
NCBI Official Full Name
proliferating cell nuclear antigen
NCBI Official Synonym Full Names
proliferating cell nuclear antigen
NCBI Official Symbol
NCBI Official Synonym Symbols
ATLD2
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NCBI Protein Information
proliferating cell nuclear antigen
UniProt Protein Name
Proliferating cell nuclear antigen
UniProt Synonym Protein Names
Cyclin
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
PCNA_HUMAN
NCBI Summary for PCNA
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
UniProt Comments for PCNA
PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.

Protein type: DNA replication; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20pter-p12

Cellular Component: centrosome; cytoplasm; DNA replication factor C complex; nuclear chromosome, telomeric region; nuclear replication fork; nucleoplasm; nucleus; PCNA complex

Molecular Function: chromatin binding; damaged DNA binding; dinucleotide insertion or deletion binding; DNA polymerase processivity factor activity; enzyme binding; histone acetyltransferase binding; identical protein binding; MutLalpha complex binding; protein binding; purine-specific mismatch base pair DNA N-glycosylase activity; receptor tyrosine kinase binding

Biological Process: bypass DNA synthesis; cell proliferation; DNA damage response, detection of DNA damage; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA strand elongation during DNA replication; epithelial cell differentiation; error-prone postreplication DNA repair; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; leading strand elongation; mismatch repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; positive regulation of deoxyribonuclease activity; positive regulation of DNA repair; positive regulation of DNA replication; protein sumoylation; replication fork processing; telomere maintenance; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair

Disease: Ataxia-telangiectasia-like Disorder 2
Product References and Citations for anti-PCNA antibody
Waseem NH & Lane DP. 1990. J Cell Sci. 96:121-9. Hall PA et al. 1990. J. Pathol. 162(4):285-94. Landberg G & Roos G. 1991. Cancer Res. 51 (17):4570-4. Woods AL et al. 1991. Histopathol. 19(1):21-7 Yu,CC. et al. 1991. Histopathol. 19(1):29-33

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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