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anti-KIT antibody :: Mouse anti-Chicken c-Kit Monoclonal Antibody

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Catalog # MBS673954
Unit / Price
  0.5 mg  /  $375 +1 FREE 8GB USB
anti-KIT antibody
Product Name

c-Kit, Monoclonal Antibody

Also Known As

Mouse Anti-Chicken c-Kit-BIOT

Product Synonym Names
Mouse Anti-Chicken c-Kit - Biotin; Mouse Anti-Chicken c-Kit
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
22
Chromosome Location
Chromosome: 4; NC_000004.11 (55524095..55606881). Location: 4q11-q12
OMIM
164920
3D Structure
ModBase 3D Structure for P10721
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgG2a kappa
Clone Number
kit2c75
Host
Mouse
Species Reactivity
Chicken
Specificity
Chicken c-Kit
c-Kit is a 145 kDa transmembrane receptor-type protein tyrosine kinase encoded by the c-Kit protooncogene and is the receptor for stem cell factor (SCF). 1 c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. 2, 3, 4, 5 The amino acid sequence of chicken c-kit shows 63% identity to mouse c-Kit and exhibits similar expression patterns in RNA blot analysis suggesting similar biological roles.1
Form/Format
Biotin (BIOT) Conjugate
Immunogen
Sp2/0 cells, Sp/chkit4A4, transfected with chicken c-kit cDNA1
Characterization
To insure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of anti-KIT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-KIT antibody
Flow Cytometry
Application Notes for anti-KIT antibody
Flow Cytometry Suggested Dilution: <=1 ug/10^6 cells
NCBI/Uniprot data below describe general gene information for KIT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
109,865 Da[Similar Products]
NCBI Official Full Name
c-Kit
NCBI Official Synonym Full Names
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
NCBI Official Symbol
NCBI Official Synonym Symbols
PBT; SCFR; C-Kit; CD117
  [Similar Products]
NCBI Protein Information
mast/stem cell growth factor receptor; OTTHUMP00000158901; OTTHUMP00000218587; proto-oncogene c-Kit; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
UniProt Protein Name
Mast/stem cell growth factor receptor
UniProt Synonym Protein Names
Proto-oncogene c-Kit; Tyrosine-protein kinase Kit
UniProt Gene Name
UniProt Entry Name
KIT_HUMAN
NCBI Summary for KIT
This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
UniProt Comments for KIT
Function: This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K).

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Interacts with APS. Interacts with MPDZ (via the tenth PDZ domain). Interacts with PTPRU. Ref.7 Ref.8 Ref.9

Subcellular location: Membrane; Single-pass type I membrane protein.

Involvement in disease: Defects in KIT are a cause of piebald trait (PBT) [

MIM:172800]. PBT is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. Ref.13 Ref.14 Ref.15 Ref.17 Ref.18 Ref.19 Ref.20 Ref.22 Ref.27Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [

MIM:606764]. Ref.19 Ref.23 Ref.24 Ref.28 Ref.29Defects in KIT have been associated with testicular tumors (TEST) [

MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms. Ref.19

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 protein kinase domain.
Product References and Citations for anti-KIT antibody
1. Sasaki, E., H. Okamura, T. Chikamune, Y. Kanai, M. Watanabe, M. Naito, and M. Sakurai. 1993. Gene. 128:257.
2. Yee, N.S., I. Paek, and P. Besmer. 1994. J. Exp. Med. 179:1994.
3. Iemura, A., M. Tsai, A. Ando, B.K. Wershil, and S.J. Galli. 1994. Am. J. Pathol. 144:321.
4. Blume-Jensen, P., L. Claesson-Welsh, A. Siegbahn, K.M. Zsebo, B. Westermark, and C.H. Heldin. 1991. EMBO J. 10:4121.
5. Kinashi, T. and T.A. Springer. 1994. Blood 83:1033.
6. Vainio, O., D. Dunon, F. Aissi, J.P. Dangy, K.M. McNagny, and B.A. Imhof. 1996. J. Cell Biol. 135:1655.
7. Lampisuo, M., K. Katevuo, and O. Lassila. 1998. Scand. J. Immunol. 48:52.
8. Lampisuo, M. J. Liippo, O. Vainio, K.M. McNagny, J. Kulmala, and O. Lassila. 1999. Int. Immunol. 11:63.
9. Schulte, C.E., M. von Lindern, P. Steinlein, H. Beug, and L.M. Wiedemann. 2002. EMBO J. 21:4297.
10. Katevuo, K., B.A. Imhof, R. Boyd, A. Chidgey, A. Bean, D. Dunon, T.W. Gobel, and O. Vainio. 1999. J. Immunol. 162:5685.

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Disclaimer
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