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Mouse anti-Chicken TCR alpha beta (V beta 1) Monoclonal Antibody

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Catalog # MBS673879
Unit / Price
  0.5 mg  /  $305 +1 FREE 8GB USB
anti-TCR alpha beta (V beta 1) antibody
Product Name

TCR alpha beta (V beta 1), Monoclonal Antibody

Popular Item
Also Known As

Mouse Anti-Chicken TCRab/Vb1-UNLB

Product Synonym Names
Mouse Anti-Chicken TCRab/Vb1 - Purified, Unlabeled Antibody; Mouse Anti-Chicken TCR alpha beta (V beta 1)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
TCR-2
Host
Mouse
Species Reactivity
Chicken
Specificity
Chicken/Turkey/Peacock/Guinea Fowl TCRalphabeta/V beta1; Mr 50& 40 kDa
Form/Format
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform with characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flow cytometer.
Preparation and Storage
Store at 2-°C.
Other Notes
Small volumes of anti-TCR alpha beta (V beta 1) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TCR alpha beta (V beta 1) antibody
MAb TCR2 precipitates a CD3-associated heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50- kDa upon reduction) on chicken peripheral blood T cells.1 Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 29-kDa from TCR2 precipitates. In the chicken, two distinct subpopulations of alpha beta T cells appear in the thymus subsequent to the appearance of gamma delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1-3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct V beta families, V beta 1 and V beta 2, respectively.4 The TCR2 MAb reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells and 40% of splenocytes in the chicken. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74+/-2%) or CD8 (26+/-4%). TCR2+ cells in the spleen also express either CD4 (37+/-1%) or CD8 (64+/-4%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2.1-3 This observation led to the demonstration that 71+/-6% of the TCR1(gamma delta)+ cells in the spleen express the CD8 marker.
Applications Tested/Suitable for anti-TCR alpha beta (V beta 1) antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
FC- Quality tested 2,9-12
IHC-FS –Reported in literature 3-5,12
IHC-PS- Reported in literature 6
IP-Reported in literature 2,12
Depletion –Reported in literature 7
Stim- Reported in literature 8



Flow Cytometry

FITC and BIOT conjugates <= 1ug/10 6 cells
PE conjugate <=0.2 ug/10 6 cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL .


Other applications :
Since applications vary, you should determined the optimum working dilution for the product that is appropriate for your specific need.
Product References and Citations for anti-TCR alpha beta (V beta 1) antibody
1. Chen, C.H., J. Cihak, U. Losch, and M.D. Cooper. 1988. Eur. J. Immunol. 18:539.
2. Chen, C.H., J.T. Sowder, J.M. Lahti, J. Cihak, U. Losch, and M.D. Cooper. 1989. Proc. Natl. Acad. Sci. (USA):86:2351
3. Char, D., P. Sanchez, C.H. Chen, R.P. Bucy, and M.D. Cooper. 1990. J. Immunol. 145:3547.
4. Chen, C.H., T.W.F. Gobel, T. Kubota, and M.D. Cooper. 1993. 1994. Poultry Science 73:1012.
5. Sowder, J.T., C.H. Chen, L.L. Ager, J. Cihak, U. Losch, and M.D. Cooper. 1988b. Fed. Proc. 2:874.
6. Cihak, J., U. Losch, G. Hoffman-Hezer, C.H. CHen, M.D. Cooper, and H.W.L. Ziegler-Heitbrock. 1993. Scand. J. Immunol. 38:123.
7. Chen, C.H. 1996. Personal communication.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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