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Mouse anti-Chicken TCR alpha beta (V beta 1) Monoclonal Antibody

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Catalog # MBS673879
Unit / Price
  0.5 mg  /  $305 +1 FREE 8GB USB
anti-TCR alpha beta (V beta 1) antibody
Product Name

TCR alpha beta (V beta 1), Monoclonal Antibody

Popular Item
Also Known As

Mouse Anti-Chicken TCRab/Vb1-UNLB

Product Synonym Names
Mouse Anti-Chicken TCRab/Vb1 - Purified, Unlabeled Antibody; Mouse Anti-Chicken TCR alpha beta (V beta 1)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Mouse IgG1 kappa
Clone Number
Species Reactivity
Chicken/Turkey/Peacock/Guinea Fowl TCRalphabeta/V beta1; Mr 50& 40 kDa
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform with characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flow cytometer.
Preparation and Storage
Store at 2-°C.
Other Notes
Small volumes of anti-TCR alpha beta (V beta 1) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TCR alpha beta (V beta 1) antibody
MAb TCR2 precipitates a CD3-associated heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50- kDa upon reduction) on chicken peripheral blood T cells.1 Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 29-kDa from TCR2 precipitates. In the chicken, two distinct subpopulations of alpha beta T cells appear in the thymus subsequent to the appearance of gamma delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1-3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct V beta families, V beta 1 and V beta 2, respectively.4 The TCR2 MAb reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells and 40% of splenocytes in the chicken. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74+/-2%) or CD8 (26+/-4%). TCR2+ cells in the spleen also express either CD4 (37+/-1%) or CD8 (64+/-4%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2.1-3 This observation led to the demonstration that 71+/-6% of the TCR1(gamma delta)+ cells in the spleen express the CD8 marker.
Applications Tested/Suitable for anti-TCR alpha beta (V beta 1) antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
FC- Quality tested 2,9-12
IHC-FS –Reported in literature 3-5,12
IHC-PS- Reported in literature 6
IP-Reported in literature 2,12
Depletion –Reported in literature 7
Stim- Reported in literature 8

Flow Cytometry

FITC and BIOT conjugates <= 1ug/10 6 cells
PE conjugate <=0.2 ug/10 6 cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL .

Other applications :
Since applications vary, you should determined the optimum working dilution for the product that is appropriate for your specific need.
Product References and Citations for anti-TCR alpha beta (V beta 1) antibody
1. Chen, C.H., J. Cihak, U. Losch, and M.D. Cooper. 1988. Eur. J. Immunol. 18:539.
2. Chen, C.H., J.T. Sowder, J.M. Lahti, J. Cihak, U. Losch, and M.D. Cooper. 1989. Proc. Natl. Acad. Sci. (USA):86:2351
3. Char, D., P. Sanchez, C.H. Chen, R.P. Bucy, and M.D. Cooper. 1990. J. Immunol. 145:3547.
4. Chen, C.H., T.W.F. Gobel, T. Kubota, and M.D. Cooper. 1993. 1994. Poultry Science 73:1012.
5. Sowder, J.T., C.H. Chen, L.L. Ager, J. Cihak, U. Losch, and M.D. Cooper. 1988b. Fed. Proc. 2:874.
6. Cihak, J., U. Losch, G. Hoffman-Hezer, C.H. CHen, M.D. Cooper, and H.W.L. Ziegler-Heitbrock. 1993. Scand. J. Immunol. 38:123.
7. Chen, C.H. 1996. Personal communication.

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