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Mouse anti-Chicken TCR gamma delta Monoclonal Antibody

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Catalog # MBS673877
Unit / Price
  0.5 mg  /  $375 +1 FREE 8GB USB
Test Data
Product Name

TCR gamma delta, Monoclonal Antibody

Popular Item
Full Product Name

Mouse Anti-Chicken TCRgd-BIOT

Product Synonym Names
T3ITCR complex, TCR gamma/delta
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgG1K
Clone Number
TCR1
Host
Mouse
Species Reactivity
Chicken
Specificity
Chicken/Milk Snake TCR gamma/delta; Mr 50 & 40 kDa
Form/Format
Biotin (BIOT) Conjugate
Immunogen
Outbred chicken thymocytes and Ig-negative blood lymphocytes
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScan flow cytometer.
Preparation and Storage
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Other Notes
Small volumes of anti-TCR gamma delta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TCR gamma delta antibody
The monoclonal antibody TCR-1 precipitates a heterodimer of Mr 90 kDa (two bands of Mr 50 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 35 kDa and 32 kDa. Unlike human and mouse in which yo cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of yo T cells. The frequency of TCRgamma/delta cells is typically 20-25% of the total blood T cells but may reach approximately 50% in chickens of six- months of age. The majority of TCRgamma/delta+ cells in the thymus and blood are CD4-CD8- although a small subset of them may express CD8 or CD4 coreceptors. However, when the TCRgamma/delta+ cells migrate into the spleen and intestine most of them begin to express CD8. Although the biological function of yo T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ yo T cells may also be involved in down-regulation of the immune response. They cannot, however, induce a graft-vs-host (GVH) reactions.
Applications Tested/Suitable for anti-TCR gamma delta antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
Application Notes for anti-TCR gamma delta antibody
Research Application(s): In ovo depletion of gamma delta T cells

Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells

Other Applications: Since application vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.

Chicken peripheral blood mononuclear cells were stained with Mouse Anti-Chicken TCRYO-PE (5B Cat. No. MBS673878) and Mouse Anti-Chicken CD3-FITC (5B Cat. No. MBS673862).
anti-TCR gamma delta antibody Test Data image
Product References and Citations for anti-TCR gamma delta antibody
1. Sowder, J.T., C.-L. Chen, L.L. Ager, M.M. Chan, and M.D. Cooper. 1988. J. Exp. Med. 167:315.
2. Chen, C.H., J. Cihak, U. Losch, and M.D. Cooper. 1988. Eur. J. Immunol. 18:539
3. Quere, P., M.D. Cooper, and G.J. Thorbecke. 1990. Immunology 71:517.
4. Chen, C.H., T.W.F. Gobel, T. Kubota, and M.D. Cooper. 1993. 1994. Poultry Science 73:1012.
5. Chen, C.-L. 1996. Personal communication.
6. Sowder, J.T., C.H. Chen, L.L. Ager, J. Cihak, U. Losch, and M.D. Cooper. 1988b. Fed. Proc. 2:874.
7. Cihak, J., U. Losch, G. Hoffman-Hezer, C.H. Chen, M.D. Cooper, and H.W.L. Ziegler-Heitbrock. 1993. Scand. J. Immunol. 38:123.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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