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anti-KRT76 antibody :: Mouse Cytokeratin, Basic Monoclonal Antibody

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Catalog # MBS438272
Unit / Price
Scan QR to view Datasheet
  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  7 mL (Prediluted)  /  $270 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

Cytokeratin, Basic (KRT76), Monoclonal Antibody

Popular Item
Full Product Name

Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Mouse Monoclonal Antibody

Product Synonym Names
KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
616671
3D Structure
ModBase 3D Structure for Q01546
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SPM116
Host
Mouse
Species Reactivity
Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken
Specificity
This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. This MAb recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Cellular Localization
Cytoplasmic
Immunogen
Human epidermal keratin
Hu-Chromosome Location
12q13.13
Positive Control
Skin, Adenocarcinomas
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-KRT76 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-KRT76 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-KRT76 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blot (0.5-1ug/ml f)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-KRT76 antibody
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Cytokeratin, HMW Monoclonal Antibody (SPM116).
anti-KRT76 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for KRT76. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8)
NCBI Official Full Name
keratin, type II cytoskeletal 2 oral
NCBI Official Synonym Full Names
keratin 76
NCBI Official Symbol
KRT76  [Similar Products]
NCBI Official Synonym Symbols
KRT2B; KRT2P; HUMCYT2A
  [Similar Products]
NCBI Protein Information
keratin, type II cytoskeletal 2 oral
UniProt Protein Name
Keratin, type II cytoskeletal 2 oral
UniProt Synonym Protein Names
Cytokeratin-2P; CK-2P; K2P; Keratin-76; K76; Type-II keratin Kb9
Protein Family
UniProt Gene Name
KRT76  [Similar Products]
UniProt Synonym Gene Names
KRT2B; KRT2P; CK-2P; K2P; K76  [Similar Products]
UniProt Entry Name
K22O_HUMAN
NCBI Summary for KRT76
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq, Jun 2009]
UniProt Comments for KRT76
K76: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleus
Product References and Citations for anti-KRT76 antibody
Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8. Tseng SCG et. al. Cell 1982; 30361

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-KRT76 antibody
 Organ/Tissue Name  Pubmed Publications
 Mouth Antibodies  >1 publications with KRT76 and Mouth
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