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anti-ACTB antibody :: Rabbit ACTB Monoclonal Antibody

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Catalog # MBS9135601
Unit / Price
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  0.05 mL  /  $180 +1 FREE 8GB USB
  0.1 mL  /  $240 +1 FREE 8GB USB
Product Name

ACTB, Monoclonal Antibody

Full Product Name

ACTB Monoclonal Antibody

Product Synonym Names
BRWS1; PS1TP5BP1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X00351 mRNA
3D Structure
ModBase 3D Structure for P60709
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Chicken, Zebrafish, Pig, Oryza sativa
Purity/Purification
Affinity Purification
Immunogen
Recombinant protein of human beta-actin
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cytoplasm, cytoskeleton
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-ACTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACTB antibody
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Product Categories/Family for anti-ACTB antibody
Applications Tested/Suitable for anti-ACTB antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ACTB antibody
WB: 1:50000 - 1:200000
IHC: 1:200 - 1:2000

Western Blot (WB) of anti-ACTB antibody
Western blot analysis of extracts of various cell lines, using beta-actin antibody at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
anti-ACTB antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ACTB antibody
Western blot analysis of extracts of HeLa cells, using beta-actin antibody at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
anti-ACTB antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ACTB antibody
Immunohistochemistry of paraffin-embedded human breast cancer using beta-actin antibody at dilution of 1:100 (20x lens).
anti-ACTB antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ACTB antibody
Immunohistochemistry of paraffin-embedded mouse liver using beta-actin antibody at dilution of 1:100 (20x lens).
anti-ACTB antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ACTB antibody
Immunohistochemistry of paraffin-embedded rat kidney using beta-actin antibody at dilution of 1:100 (20x lens).
anti-ACTB antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Calculated: 41kDa
Observed: 43kDa
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin beta
NCBI Official Symbol
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
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NCBI Protein Information
actin, cytoplasmic 1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
Protein Family
UniProt Gene Name
NCBI Summary for ACTB
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
UniProt Comments for ACTB
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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