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anti-XRCC6 (p70) antibody :: Mouse Ku (p70/p80) Monoclonal Antibody

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Catalog # MBS438044
Unit / Price
  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
anti-XRCC6 (p70) antibody
Product Name

Ku (p70/p80) (XRCC6 (p70)), Monoclonal Antibody

Popular Item
Full Product Name

Ku (p70/p80)

Product Synonym Names
Ku (p70): 70 kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku autoantigen; 70kDa; Ku70; Kup70; Lupus Ku autoantigen protein p70; ML8; Thyroid autoantigen 70kD (Ku antigen); Thyroid-lupus autoantigen (TLAA); X-ray repair cross-complementing protein 6 (XRCC6) Ku (p80): 86kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 86 Kd subunit; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; KARP1; Ku autoantigen 80kDa; Ku80; Ku86 autoantigen related protein 1; KUB2; Lupus Ku autoantigen protein p86; Nuclear factor IV (NFIV); Thyroid-lupus autoantigen (TLAA); X-ray repair cross-complementing protein 5 (XRCC5)
Product Synonym Gene Name
XRCC5 (p80)[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
152690
3D Structure
ModBase 3D Structure for P12956
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
KU729
Host
Mouse
Species Reactivity
Human, Primates. Does not react with mouse, rat and chicken.
Specificity
Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. MAb KU729 recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Localization
Nuclear
Positive Control
Human cancer K562, HL-60, HEp-2 or HeLa cells
Human Chromosomal Location
XRCC6 (p70); XRCC5 (p80)
Immunogen
Nuclear extract of human HL-60 cells
Quantity and Buffer
0.5 ml at 200ug/ml with BSA and azide
Preparation and Storage
Store at 2 to 8 degree C. Antibody is stable for 24 months.
Other Notes
Small volumes of anti-XRCC6 (p70) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-XRCC6 (p70) antibody
Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistology (IH)
Application Notes for anti-XRCC6 (p70) antibody
Immunofluorescence (0.5-1.0ug/ml); Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunocytochemistry (Acetone-fixed cells) (0.25-0.5ug/ml for 30 minutes at RT) Immunohistology (Frozen & Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
NCBI/Uniprot data below describe general gene information for XRCC6 (p70). It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
70kDa; 80kDa
NCBI Official Full Name
X-ray repair cross-complementing protein 6 isoform 1
NCBI Official Synonym Full Names
X-ray repair complementing defective repair in Chinese hamster cells 6
NCBI Official Symbol
XRCC6  [Similar Products]
NCBI Official Synonym Symbols
ML8; KU70; TLAA; CTC75; CTCBF; G22P1
  [Similar Products]
NCBI Protein Information
X-ray repair cross-complementing protein 6; 5'-dRP lyase Ku70; Ku autoantigen, 70kDa; DNA repair protein XRCC6; Ku autoantigen p70 subunit; 70 kDa subunit of Ku antigen; thyroid-lupus autoantigen p70; lupus Ku autoantigen protein p70; 5'-deoxyribose-5-phosphate lyase Ku70; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; thyroid autoantigen 70kD (Ku antigen); ATP-dependent DNA helicase 2 subunit 1; thyroid autoantigen 70kDa (Ku antigen); ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit
UniProt Protein Name
X-ray repair cross-complementing protein 6
UniProt Synonym Protein Names
5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Ku70; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6
UniProt Gene Name
XRCC6  [Similar Products]
UniProt Synonym Gene Names
G22P1; 5'-dRP lyase Ku70; CTC75; CTCBF; Ku70; TLAA  [Similar Products]
UniProt Entry Name
XRCC6_HUMAN
NCBI Summary for XRCC6 (p70)
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
UniProt Comments for XRCC6 (p70)
Ku70: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Protein type: Nuclear receptor co-regulator; DNA repair, damage; RNA-binding; DNA-binding; EC 4.2.99.-; Helicase; EC 3.6.4.-

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; transcription factor complex; nuclear telomere cap complex; membrane; nucleolus; nucleus; cytosol

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; double-stranded DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding

Biological Process: positive regulation of neurogenesis; V(D)J recombination; transcription, DNA-dependent; viral reproduction; positive regulation of transcription, DNA-dependent; DNA repair; DNA duplex unwinding; double-strand break repair via nonhomologous end joining; DNA ligation; double-strand break repair; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; telomere maintenance
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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