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Mouse beta-Actin Monoclonal Antibody

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Catalog # MBS525010
Unit / Price
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  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $220 +1 FREE 8GB USB
  0.2 mg  /  $285 +1 FREE 8GB USB
  1 mg  /  $775 +1 FREE 8GB USB
Product Name

beta-Actin, Monoclonal Antibody

Full Product Name

Anti-beta-Actin (BA3R) Loading Control Monoclonal Antibody

Product Synonym Names
Anti-beta-Actin; Anti-b-Actin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
BA3R
Host
Mouse
Species Reactivity
beta-Actin from human, mouse, rat, rabbit, and chicken. beta-Actin from other species may also be detectable
Specificity
Recognizes native and denatured forms of beta-Actin (~42kDa)
Purity/Purification
Protein A affinity chromatography from mouse ascites fluid
Immunogen
beta-Actin N-terminal peptide-KLH conjugates
Preparation and Storage
Store at -20 degree C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4 degree C (add 0.05% NaN3 if not included) for several days to weeks
Other Notes
Small volumes of anti-beta-Actin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-beta-Actin antibody
Applications Tested/Suitable for anti-beta-Actin antibody
Dot Blot (DB), ELISA (EIA), Immunostaining (IS), Western Blot (WB)
Application Notes for anti-beta-Actin antibody
WB (with ECL): 1:1000-5000 dilution (incubate for 1 hour at room temperature)

Testing Data of anti-beta-Actin antibody
Fig. 01 EBI's three loading control mAbs reacting against 10ug/lane of mouse brain tissue lysates. 50kDa band is Anti-beta-Tubulin (BT7R) at 1:2000 dilution (0.5ug/mL); 42kDa band is Anti-beta-Actin (BA3R) at 1:1000 dilution (1ug/mL); 37kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2ug/mL)
anti-beta-Actin antibody Testing Data image
Testing Data #2 of anti-beta-Actin antibody
Fig. 02 1:1000 (1ug/mL) Ab dilution used in WB of 20ug/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4)
anti-beta-Actin antibody Testing Data #2 image
NCBI/Uniprot data below describe general gene information for beta-Actin. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI Official Full Name
beta-actin, partial
Protein Family
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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