• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-Pou4f1 antibody :: Mouse Brn3A Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS601428
Unit / Price
  1 mL  /  $680 +1 FREE 8GB USB
anti-Pou4f1 antibody
Product Name

Brn3A (Pou4f1), Monoclonal Antibody

Also Known As

Brn3A (Pou Domain Transcription Factor)

Product Synonym Names
Anti -Brn3A (Pou Domain Transcription Factor)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
chromosome: 14; Location: 14; 14 E1-E3
3D Structure
ModBase 3D Structure for P17208
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q24
Host
Mouse
Species Reactivity
Chicken, Monkey
Specificity
Brn-3a. Shows no reactivity to Brn-3b or Brn-3c by Western blot. Showed no reactivity to Brn-3a knock-out mice. Species Reactivity: Monkey and chicken
Purity/Purification
Supernatant
Culture supernatant
Form/Format
Supplied as a liquid. No preservative added.
Immunogen
aa186-224 of Brn-3a fused to the T7 gene 10 protein (1).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-Pou4f1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Pou4f1 antibody
Brn3a (transcription factor 1 (brain specific homeobox/POU domain protein 3A) POU4F1, POU domain, class 4, RDC-1) is a transcription factor specifically expressed in cells of the developing mammalian nervous system.
Product Categories/Family for anti-Pou4f1 antibody
Applications Tested/Suitable for anti-Pou4f1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Pou4f1 antibody
Suitable for use in Immunohistochemistry, Western Blot.
Dilution: Immunohistochemistry: 1:5-1:125
Western blot: 1:50-1:100
NCBI/Uniprot data below describe general gene information for Pou4f1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,767 Da[Similar Products]
NCBI Official Full Name
Brn3a
NCBI Official Synonym Full Names
POU domain, class 4, transcription factor 1
NCBI Official Symbol
Pou4f1  [Similar Products]
NCBI Official Synonym Symbols
Brn3; Brn-3; Brn3a; Brn3.0; Brn-3.0; E130119J07Rik; Pou4f1
  [Similar Products]
NCBI Protein Information
POU domain, class 4, transcription factor 1; brn-3A; brain-3A; brain-specific homeobox/POU domain protein 3A
UniProt Protein Name
POU domain, class 4, transcription factor 1
UniProt Synonym Protein Names
Brain-specific homeobox/POU domain protein 3A; Brain-3A; Brn-3A; Brn-3.0
UniProt Gene Name
Pou4f1  [Similar Products]
UniProt Synonym Gene Names
Brn-3; Brn3; Brn3a  [Similar Products]
UniProt Entry Name
PO4F1_MOUSE
UniProt Comments for Pou4f1
POU4F1: Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Belongs to the POU transcription factor family. Class- 4 subfamily.

Protein type: DNA-binding; Cell development/differentiation; Transcription factor

Cellular Component: neuron projection; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; nervous system development; trigeminal nerve development; central nervous system neuron differentiation; transcription, DNA-dependent; positive regulation of apoptosis; multicellular organismal development; peripheral nervous system neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; habenula development; regulation of transcription from RNA polymerase II promoter; cell migration in hindbrain; neuron differentiation; peripheral nervous system neuron development; proprioception during equilibrioception; regulation of neurogenesis; regulation of transcription, DNA-dependent; suckling behavior; neuron fate specification; mesoderm development; positive regulation of transcription from RNA polymerase II promoter; sensory system development; negative regulation of neuron apoptosis
Research Articles on Pou4f1
1. data define AR as a direct Brn-3a interactor and verify a simple interacting protein prediction methodology that is likely to be useful for many other proteins
2. Data show that Brn3a acts upstream of the Runx factors, which then repress TrkB expression to allow establishment of the non-overlapping Trk receptor profiles and correct terminally differentiated phenotypes.
3. Brn3a regulates the transition from neurogenesis to terminal differentiation and represses non-neural gene expression in the trigeminal ganglion.
4. these findings begin to define a gene regulatory pathway for habenula development in mammals, and the roles of Nurr1 and Brn3a therein.
5. Brn-3a deficiency affects the number and cell size of CGRP- and calcium-binding protein-containing neurons.
6. Loss of Brn3a leads to an increase in the ratio of bistratified to monostratified RGCs, with only modest RGC loss and little effect on central projections.
7. Brn3a represses differences in gene expression between sensory neurons generated at different axial levels, and regulates developmental gene expression; functionally similar populations of neurons are generated from different embryological substrates.
8. results suggest that co-factors binding to p73 C terminus facilitate activation on Bax but not p21 CIP1/Waf1 promoter and that Brn-3a modulates this interaction
9. Data suggest that Brn-3a and b POU factors may play an important role in the development and maintenance of critical cell types and structures within the heart, in part via developmental regulation of myocardial Hsp27 expression.
10. Data show that Pou4f1 is regionally enriched in the Brainstem (Pons and Medulla) of mouse brain.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000