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Bu-1b Secondary Antibody

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Catalog # MBS673951
Unit / Price
  0.1 mg  /  $285 +1 FREE 8GB USB
Bu-1b secondary antibody
Product Name

Bu-1b, Secondary Antibody

Full Product Name

Mouse Anti-Chicken Bu-1b-PE

Product Synonym Names
Mouse Anti-Chicken Bu-1b - Phycoerythrin-Labeled; Mouse Anti-Chicken Bu-1b
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgG1 kappa
Clone Number
5-11G2
Host
Mouse
Species Reactivity
Chicken
Specificity
Chicken Bu-1b alloantigen, Mr 70 kDa
Chicken Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 5-11G2 reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa.1-2
Form/Format
R - phycoerythrin (R - PE) Conjugate
Immunogen
Bursal cells from 2- to 5-week-old CHA (Bu-1b) strain chickens 1
Characterization
To insure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are shown on page 2 of this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of Bu-1b secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for Bu-1b secondary antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
Application Notes for Bu-1b secondary antibody
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Product References and Citations for Bu-1b secondary antibody
1. Veromaa, T., O. Vainio, E. Eerola, and P. Toivanen. 1988. Hybridoma 7:41.
2. Houssaint, E., E. Diez, and J.R. Pink. 1987. Immunol. 62:463.
3. Vainio, O. 1998. Personal communication.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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