• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-CYCS antibody :: Mouse Cytochrome C Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS439344
Unit / Price
Scan QR to view Datasheet
  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  7 mL (Prediluted)  /  $270 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
Product Name

Cytochrome C (CYCS), Monoclonal Antibody

Popular Item
Full Product Name

Cytochrome C (Mitochondrial Marker) Mouse Monoclonal Antibody

Product Synonym Names
CYC; CYCS; HCS; THC4
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
123970
3D Structure
ModBase 3D Structure for P99999
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
7H8.2C12
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Horse, Dog, Pigeon, Frog, Drosophila.
Others not known
Specificity
It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Cellular Localization
Cytoplasmic
Immunogen
Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
Hu-Chromosome Location
7p15.3
Positive Control
K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-CYCS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CYCS antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-CYCS antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-CYCS antibody
Formalin-fixed, paraffin-embedded human Salivary Tumor stained with Cytochrome C Monoclonal Antibody (7H8.2C12).
anti-CYCS antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CYCS antibody
Formalin-fixed, paraffin-embedded human Pancreas stained with Cytochrome C Monoclonal Antibody (7H8.2C12).
anti-CYCS antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CYCS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15kDa
NCBI Official Full Name
cytochrome c
NCBI Official Synonym Full Names
cytochrome c, somatic
NCBI Official Symbol
NCBI Official Synonym Symbols
CYC; HCS; THC4
  [Similar Products]
NCBI Protein Information
cytochrome c
UniProt Protein Name
Cytochrome c
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
CYC_HUMAN
NCBI Summary for CYCS
This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
UniProt Comments for CYCS
CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family.

Protein type: Mitochondrial; Oxidoreductase; Protein phosphatase, Ser/Thr (non-receptor); Apoptosis

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrion; nucleus; protein phosphatase type 2A complex

Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; heme binding; protein binding; protein serine/threonine phosphatase activity

Biological Process: caspase activation via cytochrome c; cellular respiration; mitochondrial electron transport, cytochrome c to oxygen; mitochondrial electron transport, ubiquinol to cytochrome c; mitochondrion organization and biogenesis; response to reactive oxygen species

Disease: Thrombocytopenia 4
Product References and Citations for anti-CYCS antibody
Goshorn SG, E Retzel, and R Jemmerson. Common Structural Features among Monoclonal Antibodies Binding the Same Antigenic Region of Cytochrome c. J Biol Chem 266:2134-2142 (1991).

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000