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anti-GAPDH antibody :: Mouse GAPDH Monoclonal Antibody

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Catalog # MBS462002
Unit / Price
  0.1 mg  /  $285 +1 FREE 8GB USB
Testing Data
Product Name

GAPDH, Monoclonal Antibody

Popular Item
Also Known As

Mouse anti GAPDH antibody

Product Synonym Names
Mouse anti-GAPDH
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 4; NC_005103.2 (161282215..161286090, complement). Location: 4q42
3D Structure
ModBase 3D Structure for P04797
Clonality
Monoclonal
Clone Number
6C5
Host
Mouse
Species Reactivity
Human, Rat, Mouse, Dog, Fish
Specificity
This antibody recognizes Glyceraldehyde-3-PDH monomer (36 kDa) and the dimer form. It reacts with human, rabbit, rat, mouse, dog, cat, fish and pig. It does not react with GAPDH from bovine, goat and yeast.
Purity/Purification
The Mouse IgG1 is purified by Epitope Affinity Purification
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
Antigen Preparation
A purified rabbit muscle GAPDH (whole molecule).
Positive Control
Rabbit Muscle
Cellular Location
Cytoplasmic
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Other Notes
Small volumes of anti-GAPDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-GAPDH antibody
ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Application Notes for anti-GAPDH antibody
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml

Testing Data of anti-GAPDH antibody
anti-GAPDH antibody Testing Data image
NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
36 kDa (monomer)
NCBI Official Full Name
Glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
Gapdh  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase; BARS-38; peptidyl-cysteine S-nitrosylase GAPDH; 38 kDa BFA-dependent ADP-ribosylation substrate
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Synonym Protein Names
38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH
UniProt Gene Name
Gapdh  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
G3P_RAT
NCBI Summary for GAPDH
catalyzes the interconversion of D-glyceraldehyde 3-phosphate, phosphate, and NAD(+) to 3-phospho-D-glyceroyl phosphate and NADH; may contribute to apoptosis [RGD]
UniProt Comments for GAPDH
Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Ref.3 Ref.13 Ref.15

Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure: Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct

By similarity. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Ref.13 Ref.14 Ref.15

Subcellular location: Cytoplasm › cytosol. Nucleus. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Ref.13 Ref.14 Ref.15

Tissue specificity: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney. Ref.9

Post-translational modification: S-nitrosylation of Cys-150 leads to interaction with SIAH1, followed by translocation to the nucleus. The effect of S-nitrosylation on enzymatic activity is unclear: according to some authors, it inhibits enzymatic activity and increases endogenous ADP-ribosylation, inhibiting the enzyme in a non-reversible manner (Ref.12). According to others, it does not affect glycolysis (Ref.13). ADP-ribosylation is likely to be a pathophysiological event associated with inhibition of gluconeogenesis.ISGylated

Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withanti-GAPDH antibody
 Reference Product  PubMed Publications
 Hpcal1 antibody  >1 publications with GAPDH and Hpcal1
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